Team:Waterloo/Notebook/strainlist

From 2009.igem.org

Strain list

LocationStrain #NameChassis/StrainPlasmid BackboneRegistry PartAntibiotic ResistanceDescriptionReference Date
iGEM strain box 1 1 rTT + PocI DH5α pMA (GeneArt) [http://partsregistry.org/Part:BBa_K093003 BBa_K093003] Amp100 Synthesized. Contains BioBrick prefix and suffix. Reverse orientation transcriptional terminator R0015 with λ Pr promotor. 2008:09:09
iGEM strain box 1 2 rPlacTT DH5α pMA (GeneArt) [http://partsregistry.org/Part:BBa_K093004 BBa_K093004] Amp100 Synthesized. Contains BioBrick prefix and suffix. Reverse [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] and transcriptional terminator [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]. 2008:09:09
iGEM strain box 1 3 PmeI DH5α pMA (GeneArt) [http://partsregistry.org/Part:BBa_K093002 BBa_K093002] Amp100 Synthesized ORF. Contains BioBrick prefix and suffix. 2008:09:09
iGEM strain box 1 4 rfp'-14 DH5α pSB1A2 [http://partsregistry.org/Part:BBa_K093005 BBa_K093005] Amp100 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) colony 14. 2008:09:09
iGEM strain box 1 5 rfp-2 DH5α pSB1AK3 Amp100, Kan25 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) colony 2. Might need testing 2008:09:09
iGEM strain box 1 6 Pconst-rfp-1 DH5α pSB1A2 [http://partsregistry.org/Part:BBa_K093012 BBa_K093012] Amp100 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) under a constitutive promoter ([http://partsregistry.org/Part:BBa_J23118 BBa_J23118]). Might need testing. 2008:09:09
iGEM strain box 1 7 pRecA-3 DH5α psb2k3 [http://partsregistry.org/Part:BBa_K093000 BBa_K093000] Kan25 Engineered RecA promoter with lexA consensus sequence. 2008:10:17
iGEM strain box 1 8 Pconst-rfp' 1 DH5α psb1a2 Amp100 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) under a constitutive promoter ([http://partsregistry.org/Part:BBa_J23118 BBa_J23118]) 2008:10:17
iGEM strain box 1 9 c0051' DH5α psb1a2 [http://partsregistry.org/Part:BBa_K093006 BBa_K093006] Amp100 [http://partsregistry.org/Part:BBa_C0051 BBa_C0051] Repressor for Lambda Pr promoter with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]). Never got sequencing results of prefix. Rest was good 2008:10:17
iGEM strain box 1 10 PmeI - 14 DH5α psb2k3 [http://partsregistry.org/Part:BBa_K093002 BBa_K093002] Kan25 PmeI cloned into BioBrick vector 2008:10:17
iGEM strain box 1 11 PrecA RFP' DH5α pSB2K3 [http://partsregistry.org/Part:BBa_K093009 BBa_K093009] Kan25 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) under the control of [http://partsregistry.org/Part:BBa_K093000 BBa_K093000] promoter 2008:10:17
iGEM strain box 1 12 PmeI' DH5α pSB1A2 [http://partsregistry.org/Part:BBa_K093013 BBa_K093013] Amp100 PmeI ([http://partsregistry.org/Part:BBa_K093002 BBa_K093002]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) 2008:11:04
iGEM strain box 1 13 Pr RFP' DH5α psb1a2 [http://partsregistry.org/Part:BBa_K093010 BBa_K093010] Amp100 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) under Lamda Pr promoter (R0051) 2008:11:04
iGEM strain box 1 14 T7 gene 6 DH5α pSB1A2 [http://partsregistry.org/Part:BBa_K093001 BBa_K093001] Amp100 good one, correct stop codon taataa 2009:02:13
iGEM strain box 1 15 PkNG101 DH5α λ pir Strep100 Only replicates in λ pir lysogen (r6k origin of replication) http://bccm.belspo.be/db/lmbp_plasmid_details.php?NM=pKNG101 2009:02:xx
iGEM strain box 1 16 H1-4 pJET DH5α pJET Amp100 E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream 2009:03:18
iGEM strain box 1 17 CI pJET DH5α pJET Amp100 CI repressor for lamda Pr promoter. [http://partsregistry.org/Part:BBa_C0051 BBa_C0051] with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) in pJET (made by crossover PCR) 2009:03:xx
iGEM strain box 1 18 pFB9002 DH5α lamda pir pfb9001 Strep 100 E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream 2009:03:xx
iGEM strain box 1 19 pJET H2 DH5α pJET Amp100 E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream 2009:04:09
iGEM strain box 1 20 Pfb9003 DH5α lamda pir pfb9001 Strep100 E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream 2009:04:23
iGEM strain box 1 21 pfb9004 DH5α lamda pir pfb9001 Strep100 Contains two attP sites flanked by E. coli melA genomic DNA for homologous recombination (H1 and H2) 2009:04:23
iGEM strain box 1 22 pJET rfp-1 DH5α pJET Amp100 insert: Pconst ([http://partsregistry.org/Part:BBa_J23118 BBa_J23118]) rfp ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) EcoRV fragment for propagating pJET 2009:04:24
iGEM strain box 1 79 pJET rfp-2 DH5α pJET Amp100 insert: Pconst rfp EcoRV fragment 2009:04:24
iGEM strain box 1 81 pJET rfp-3 DH5α pJET Amp100 insert: Pconst rfp EcoRV fragment 2009:04:24
iGEM strain box 1 23 RBS DH5α pSB1A2? B0034 Amp100 Ribosome binding site (Elowitz)
iGEM strain box 1 24 Pconst55% DH5α [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] [http://partsregistry.org/Part:BBa_J23118 BBa_J23118] Amp100 Constitutive promoter. Expression level is 55% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)
iGEM strain box 1 25 TT DH5α pSB1AK3 [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Amp100, Kan25 Default double transcriptional terminator
iGEM strain box 1 26 rfp DH5α pSB2K3 E1010 Kan25 Red fluorescent protein (shows up pink even without appropriate wavelength of light and filter)
iGEM strain box 1 27 Pseudomonas mendocina kr 1 ? Wrong strain for cloning PmeI (received from Paul Hatzinger at Shaw Environmental NJ). Degrades N-nitrodimethylamine (NDMA). Appl Environ Microbiol. 2006 Oct;72(10):6693-8. Epub 2006 Sep 1 2008:08:xx
iGEM strain box 1 28 Plac DH5α pSB1A2 [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Amp100 Wildtype LacI repressable promoter
iGEM strain box 1 29 PtetR DH5α pSB1A2 [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] Amp100 Promoter that is repressed by tetR and derepressed by tetracyclin.
iGEM strain box 1 30 CI DH5α pSB1A2 [http://partsregistry.org/Part:BBa_C0051 BBa_C0051] Amp100 CI repressor for λ Pr promoter
iGEM strain box 1 31 rTT DH5α pSB1A2 B0025 Amp100 Reverse transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) 2008:xx:xx
iGEM strain box 1 32 Taq DH5α Amp100 Taq strain from Moffatt lab for purifying taq 2008:08:xx
iGEM strain box 1 33 Pconst+ DH5α pSB1A2 [http://partsregistry.org/Part:BBa_J23119 BBa_J23119] Amp100 Wildtype promoter─strongest of this promoter family ([http://partsregistry.org/Part:BBa_J23100 BBa_J23100]-[http://partsregistry.org/Part:BBa_J23119 BBa_J23119])
iGEM strain box 1 34 LacI DH5α pSB1A2 [http://partsregistry.org/Part:BBa_C0012 BBa_C0012] Amp100 LacI repressor for Plac Promoter ([http://partsregistry.org/Part:BBa_R0010 BBa_R0010]) with LVA degredation tag
iGEM strain box 1 35 Pconst4% DH5α [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] Amp100 Constitutive promoter. Expression level is 4% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)
iGEM strain box 1 36 TTbi DH5α pSB1AK3 [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] Amp100,Kan25 Double terminator─not as strong as [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] but more even efficiency for forward and reverse use.
iGEM strain box 1 37 Pconst100% DH5α [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] Amp100 Constitutive promoter. Expression level is 100%─the highest expression level for this Pconst family (i.e., wildtype promoter)
iGEM strain box 1 38 PlacCI DH5α pSB2K3 Kan25 cI repressor ([http://partsregistry.org/Part:BBa_C0051 BBa_C0051]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) under the promoter Plac ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011]). 2009:04:xx
iGEM strain box 1 39 pTC300 DH5α pUC19 Cm10, Kan25 Strain containing Kanamycin resistance gene, and SacB negative selection gene flanked together by BamHI sites 2009:05:30
iGEM strain box 1 40 DB 3.1 DB 3.1 CCDB resistant strain, contains mutation in GyrA 2009:05:30
iGEM strain box 1 41 DH5α DH5 alpha For making competent cells. Default strain for 2007:xx:xx
iGEM strain box 1 42 pFB9006 DH5α λ pir pFB9001 Strep100/Kan20 pFB9005+KanSac. pFB9001 containing H2 homologous region to E. coli melA gene. Does not contain H1 region. 2009:06:xx
iGEM strain box 1 43 pSB1A3 DH5α pSB1A3 Amp 100 BioBrick vector from 2009 registry. 2009:06:16
iGEM strain box 1 44 luxI DH5α pSB1AK3 Amp100, Kan 25?? luxI ([http://partsregistry.org/Part:BBa_C0061 BBa_C0061]) +RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) 2007:xx:xx
iGEM strain box 1 45 PlaclasR DH5α pSB1A2 Amp100 lasR (C0079) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) under the control of a lac promoter (unsure which promoter - would need testing/sequencing) 2007:xx:xx
iGEM strain box 1 46 Plux rfp' DH5α pSB2K3 Kan25 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) under Plux promoter 2007:xx:xx
iGEM strain box 1 47 Plas rfp' DH5α pSB2K3 Kan25 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) under Plas promoter ([http://partsregistry.org/Part:BBa_R0079 BBa_R0079]) 2007:xx:xx
iGEM strain box 1 48 Ptet rfp' DH5α pSB2K3 Kan25 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) under Ptet promoter ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]) 2007:xx:xx
iGEM strain box 1 49 PompR rfp' DH5α pSB2K3 Kan25 RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) under PompR promoter 2007:xx:xx
iGEM strain box 1 50 tetR DH5α pSB2K3 Kan25 tetR repressor ([http://partsregistry.org/Part:BBa_C0040 BBa_C0040]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) 2007:xx:xx
iGEM strain box 1 51 ??? DH5α pSB2K3 Kan25
iGEM strain box 1 52 lasI DH5α pSB2K3 Kan25 lasI ([http://partsregistry.org/Part:BBa_C0078 BBa_C0078]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) 2007:xx:xx
iGEM strain box 1 53 tetR DH5α pSB2K3 Kan25 tetR repressor ([http://partsregistry.org/Part:BBa_C0040 BBa_C0040]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) 2007:xx:xx
iGEM strain box 1 54 tetR' DH5α pSB1A2 Amp100 tetR repressor ([http://partsregistry.org/Part:BBa_C0040 BBa_C0040]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) 2007:xx:xx
iGEM strain box 1 55 lasR DH5α pSB1A2 Amp100 lasR ([http://partsregistry.org/Part:BBa_C0079 BBa_C0079]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) 2007:xx:xx
iGEM strain box 1 56 Plac IA7K DH5α pSB1A2 Amp100 ????
iGEM strain box 1 57 pSB1A2 attB TG DH5α pSB1A2 Amp100 Was previously Pr RFP', RFP was cut out and attB TG was inserted. Flanked by EcoRI and PstI sites. 2009:07:06
iGEM strain box 1 58 pSB1A2 attB TT DH5α pSB1A2 Amp100 Same as Strain #57, except the inserted fragment was attB TT instead. This is the wild-type attB. 2009:07:06
iGEM strain box 1 59 pSB1A2 attB CT DH5α pSB1A2 Amp100 Same as Strain #57, except the inserted fragment was attB CT instead. 2009:07:06
iGEM strain box 1 60 rPlacTT + PlacCI DH5α pSB2K3 2009:06:15
iGEM strain box 1 61 2008 Construct w/o Promoter DH5α pSB1A2 Amp100 RBS + Pmel + rPlacTT + PlacCI. 2008 project. 2009:07:10
iGEM strain box 1 62 2008 Constant DH5α Amp100 2009:07:15
iGEM strain box 1 63 pSB3C5 DB3.1 pSB3C5 Cm25 Low copy # plasmid, no promoter, no RBS, "CCDD" (BBa 15001/15002)?
iGEM strain box 1 64 BBa pSB3C5
iGEM strain box 1 65 pKNG101 DH5α pKNG101 Strep100 Plasmid backbone for the pFB9xxx series. 2009:08:07
iGEM strain box 1 66 DH5α λ pir DH5 alpha lambda pir None None DH5α λ pir stock for making competent cells. 2009:07:24
iGEM strain box 1 67 pFB9007 DH5α λ pir pFB9001 Strep100 Contains H2 region and an attP site. Same as pFB9004 except w/o the truncated SacB gene on the plasmid backbone. 2009:08:05
iGEM strain box 1 68 pFB9008 DH5α λ pir pFB9007 Strep100 Same as pFB9007, but it has RFP cloned in upstream of the attP site and the H2 region. 2009:08:05
iGEM strain box 1 69 pFB9009 #2 DH5α λ pir pFB9008+H1 Strep100/Kan20 Same as pFB9008+H2, but it has Kanr and sacB genes cloned in between the H1&H2 regions, directly downstream adjacent to the RFP. 2009:08:28
iGEM strain box 1 70
iGEM strain box 1 71 pSB4A5 DH5α pSB4A5 Amp100 Low copy number, no promoter, no RBS. 2009:08:28
iGEM strain box 1 72 pSB4C5 DB3.1 pSB4C5 Cm25 Low copy number, no promoter, no RBS. 2009:08:28
iGEM strain box 1 73 OriT-L #13 DH5α pSB1A2 Amp100 2009:08:28
iGEM strain box 1 74 OriT-L #18 DH5α pSB1A2 Amp100 2009:08:28
iGEM strain box 1 75 [http://partsregistry.org/Part:BBa_J23116 BBa_J23116] Pconst DH5α [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] Amp100 2009:08:28
iGEM strain box 1 76 [http://partsregistry.org/Part:BBa_J23118 BBa_J23118] Pconst DH5α [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] Amp100 2009:08:28
iGEM strain box 1 77 pFB9008+H1 #13 DH5α λ pir pFB9007 Strep100/Kan20 Same as pFB9008, except that H1 region and its attP site are cloned in upstream of the RFP gene to flank the RFP. Low copy number. 2009:08:28
iGEM strain box 1 78 pSB4C5-1 DB3.1 pSB4C5 Cm25 2009:08:20
iGEM strain box 1 79 pSB4C5-2 DB3.1 pSB4C5 Cm25 2009:08:20