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Template:Team:KULeuven/26 August 2009/BlueLightReceptor
From 2009.igem.org
- The plates with ligation A (blp + GFP) were fetched from the blue light installation. There was no GFP signal. The following actions will be taken:
- The plasmids will be purified from the colonies and will be sequenced using primer 2260.
- Next time, we will probably put them in liquid cultures under the LED's while shaking gently. Also, other parameters that need to be considered are being researched.
- They will not be exposed to the light as long anymore. We decided that 1h will be more than enough.
- Possible bleaching?
- A colony from all three plates with the LigA-construct was taken and ented in liquid culture. This was needed to check whether the colonies were still alive.
- culture 13/08
- culture 14/08 (1)
- culture 14/08 (2)
- By 5pm, one of the liquid cultures had grown and was miniprepped. 20μl was sent for sequencing together with 5μM of primer 2260.
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| LigA (14/08 (1)) | 23,1 | 2,21 |
4. Two electroporations were performed and plated on LB medium. One with LigC ( + ) and one with DNA.
