ULB/11 August 2009

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iGEM Team:ULB-Brussels Wiki


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Start of construction (ligation) in Standard 10.

1st building: the promoter (BBa_R0011) and RBS (BBa_B0034)in a plasmid destination which contain the brig CcdB (BBa_P1010) (it induces cell death) and a resistance gene. As our plasmids are resistant to ampicillin, the plasmid is chosen destination pSB1C3 with brick BBa_P1010( resistance to chloramphenicol).


Laboratory manipulation

  • Thermoporation of BBa_I51020 (plasmid destination resistant to ampicillin) with thermo-skill bacteria thermocomp√©tentes.
  • Digestion of BBa_I51020 with EcoRI and PstI.

Computer part

  • Order of primers for amplification ccdA + its weak constitutive promoter (promoter mob, not from E. Coli)

Creating BioBricks (CCDA in the forward and reverse direction).