Team:Wash U/Calendar-Home/7 July 2009

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== Tuesday July 7, 2009 ==
- Ran [https://static.igem.org/mediawiki/2009/f/f0/7-7_ligation_products.jpg Gel] of digestion and ligation products from Biobrick Assembly <br>

- Performed colony PCR on colonies transformed with plasmid from ligation reaction <br>
Results: [https://static.igem.org/mediawiki/2009/b/b6/Colony_PCR_7-8.jpg Gel] [https://static.igem.org/mediawiki/2009/9/9f/8_colonies_from_colony_PCR.jpg Colonies] <br>
Red colonies indicate incorrect ligation <br>

- Made [https://2009.igem.org/Team:Wash_U/Protocol#Glycerol_Stock_Solution glycerol stock solutions]: <br>
1.  pSB1AC3 <br>
2.  puc19 E. coli <br>
3.  BBa_I15010 pSB2K3 <br>
4.  S17-1 E. coli <br>
5.  BBa_R0082 pSB1A2 <br>
6.  pSB1T3 <br>
7.  BBa_E0240 pSB1A2 <br>
8.  BBa_B0034 pSB1A2 <br>
9.  pSB1AT3 <br>
10. pRKCBC3 <br>
11. pSB1A3 <br>
12. BBa_B0015 pSB1AK3 <br>
13. pucHS27 <br>
14. pSB1K3 <br>
15. R. sphaeroides 2.4.1 <br>

-Purified genomic DNA of E. coli and R. sphaeroides for PCR <br>
DNA Quantities: <br>
1. E.coli (pellet from 1.5 mL of culture): 54.5 ng/uL <br>
2. E.coli (pellet from 3.0 mL of culture): 70.0 ng/uL <br>
3. R.sphaeroides (from 3.0 mL of culture): 62.5 ng/uL <br>
4. R.sphaeroides (from 1.5 mL of culture): 40.5 ng/uL <br>





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