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| ='''Notebook Flow Cytometry'''= | | ='''Notebook Flow Cytometry'''= |
- | =='''Contents'''==
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- | == 8-28-2009 ==
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- | * First Flow cytometry measurement with cells transfected with cmv-GFP, jet-GFP and jet-cmv-GFP using jet and cmv as reference
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- | == 9-9-2009 ==
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- | * Second FC measurement with U2OS, MCF7 and HeLa with cmv-GFP, jet-GFP and jet-cmv-GFP using jet and cmv as reference
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- | * First measurement of inducible promoters in HeLa
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- | == 9-11-2009 ==
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- | * Third FC measurement with U2OS, MCF7 and HeLa with cmv-GFP, jet-GFP and jet-cmv-GFP using only jet as reference
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- | {|
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- | |-valign="top" border="0"
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- | |width="400px" style="padding: 0 20px 0 0;"|
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- | [[image:HD09_Hannah_FACSHeLa110909.JPG|center|400px|thumb|'''Figure 1: Flow cytometry results of MCF7 GFP fluorescence intensity normalized with mCherry transfected in a 1:2 ratio.]]
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- | [[image:HD09_Hannah_HeLa110909.JPGâ|center|400px|thumb|'''Figure 2: Flow cytometry results of MCF7 GFP fluorescence intensity normalized with mCherry transfected in a 1:2 ratio.]]
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- | |width="400px"|
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- | [[image:HD09_Hannah_FACSU2OS110909.JPG|center|400px|thumb|'''Figure 3: Flow cytometry results of U2OS GFP fluorescence intensity normalized with mCherry transfected in a 1:2 ratio.]]
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- | [[image:HD09_Hannah_U2OS110909.JPG|center|400px|thumb|'''Figure 4: Flow cytometry results of U2OS GFP fluorescence intensity normalized with mCherry transfected in a 1:2 ratio.]]
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- | |}
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- | == 9-21-2009 ==
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- | * We decided to change our GFP:Cherry ratio from 1:2 to 2:1 to ensure that we have GFP in every cell expressing mCherry and thereby increasing the number of mCherry cells that also contain GFP.
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- | * Time course measurement of 24 and 96 well plate. Triplicates of the same transfection reaction were loaded at different positions in the plate to check wether the results change during the time course of the experiment.
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- | {|
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- | |-valign="top" border="0"
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- | |width="400px" style="padding: 0 20px 0 0;"|
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- | [[image:HD09_Hannah_24wellloadingscheme.JPG |center|thumb|600px|'''Figure 1: Flow cytometry results of HeLa GFP fluorescence intensity normalized with mCherry transfected in a 2:1 ratio.]]
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- | [[image:HD09_Hannah_24wellTime.JPG|center|thumb|400px|'''Figure 2: Time measurement 24 Well''' Flow cytometry results of HeLa GFP fluorescence intensity normalized with mCherry transfected in a 1:2 ratio.]]
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- | |width="400px"|
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- | [[image:HD09_Hannah_96wellloadingscheme.JPG|center|thumb|300px|'''Figure 3: Flow cytometry results of HeLa GFP fluorescence intensity normalized with mCherry transfected in a 1:2 ratio.]]
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- | [[image:HD09_Hannah_96wellTime.JPG |center|400px|thumb|'''Figure 4: Time measurement 96 Well''' Flow cytometry results of HeLa GFP fluorescence intensity normalized with mCherry transfected in a 1:2 ratio.]]
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- | |}
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| == 9-24-2009 == | | == 9-24-2009 == |