Notebook Flow Cytometry

9-24-2009

• We performed standard measurements in HeLa, U2OS and MCF7 with our three standard promoters: CMV, JeT and JeT-CMV. These measurements will be performed in 3 independent measurements. The initial result of U2OS is shown below, which includes the following samples (see table 1).

Sample negative control (transfection with a non-fluorescent plasmid p6) cotransfection with JeT plasmid and p6 cotransfection with JeT_CMV plasmid and p6 cotransfection with CMV plasmid and p6 cotransfection with mCherry reference plasmid and p6 cotransfection with CMV plasmid and mCherry reference plasmid cotransfection with JeT plasmid and mCherry reference plasmid cotransfection with JeT_CMV plasmid and mCherry reference plasmid

Figure ?: Flow cytometry results of the U2-OS standard plate The .

• We measured the constitutive promoters in the HeLa cell line. Unfortunately, the number of the viable cells are not enough for a reliable measurement.

9-25-2009

• We measured the MCF-7 Standard plate, which includes the same samples as above (see table 1)

Figure ?: Flow cytometry results of the MCF-7 standard plate The .

9-28-2009

• We measured the MCF-7 and the U2-OS Standard plate again (same samples).

Figure ?: Flow cytometry results of the U2-OS standard plate The .

Figure ?: Flow cytometry results of the MCF-7 standard plate The .

9-29-2009

• We measured the MCF-7 and the U2-OS Standard plate the third time (same samples).

Figure ?: Flow cytometry results of the U2-OS standard plate The .

Figure ?: Flow cytometry results of the MCF-7 standard plate The .

9-30-2009

• We measured the constitutive promoters in the HeLa cell line again.

10-02-2009

• We measured the U2-OS NFkB plate 10 hours after induction.

Figure ?: Flow cytometry results of the U2-OS standard plate The .

10-03-2009

• We measured the HIF promoter in MCF-7. Unfortunately, the cell number was too low.

10-05-2009

• We measured the HeLa Standard plate in three different experiments????, which includes the same samples as above (see table 1)
• We measured the constitutive promoters in the HeLa cell line again. Unfortunately, the number of the viable cells are not enough for a reliable measurement.
• We measured the AhR ??? promoter in HeLa. Unfortunately, the number of the viable cells are not enough for a reliable measurement.

Figure ?: Flow cytometry results of the U2-OS standard plate The .

Figure ?: Flow cytometry results of the MCF-7 standard plate The .

Figure ?: Flow cytometry results of the MCF-7 standard plate The .

10-07-2009

• We measured the U2-OS NFkB plate 10 hours after induction again.

Figure ?: Flow cytometry results of the U2-OS standard plate The .

10-08-2009

• We measured the Estrogen promoter in MCF-7. Unfortunately, the number of the viable cells are not enough for a reliable measurement.

10-09-2009

• We measured the HeLa Standard plate (fourth time).
• We measured the SREBP ??? in HeLa.
• We measured the constitutive promoters in HeLa again.

Figure ?: Flow cytometry results of the U2-OS standard plate The .

Figure ?: Flow cytometry results of the MCF-7 standard plate The .

Figure ?: Flow cytometry results of the MCF-7 standard plate

10-12-2009

• We measured the HeLa Standard plate (fifth time).
• We measured the promosing NKkB promoter NIIL10 ??? in different medium in U2-OS. Here, we want to see, if there is an induction of a nonspecific drug (Thiazolidin??) and how the different medium affect the promoter activity. No significant change.

Figure ?: Flow cytometry results of the U2-OS standard plate The .

Figure ?: Flow cytometry results of the MCF-7 standard plate The .

10-13-2009

• We measured the p53 ?? promoter in MCF-7. Unfortunately, there were some problems with the flow cytometry analysis.

10-14-2009

• We measured