Team:Heidelberg/Notebook measure/NotebookFC
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(Difference between revisions)
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* Time course measurement of 24 and 96 well plate. Triplicates of the same transfection reaction were loaded at different positions in the plate to check wether the results change during the time course of the experiment. | * Time course measurement of 24 and 96 well plate. Triplicates of the same transfection reaction were loaded at different positions in the plate to check wether the results change during the time course of the experiment. | ||
+ | {| | ||
+ | |-valign="top" border="0" | ||
+ | |width="450px" style="padding: 0 20px 0 0;"| | ||
+ | [[image:HD09_FACS_Time24well.PNG|center|400px|thumb|'''Figure ?: Time measurement 24 Well''' The .The standard deviations of the replicates are also shown.]] | ||
+ | |width="450px"| | ||
+ | [[image:HD09_FACS_Time96well.PNG|center|400px|thumb|'''Figure ?: Time measurement 96 Well''' The .The standard deviations of the replicates are also shown.]] | ||
+ | |} | ||
== 9-24-2009 == | == 9-24-2009 == | ||
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|width="400px"| | |width="400px"| | ||
- | [[image:HD09_2409_U2os.png|center|440px|thumb|'''Figure ?: Flow cytometry results of the U2-OS Standard plate (1st measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the U2-OS cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above. ]] | + | [[image:HD09_2409_U2os.png|center|440px|thumb|'''Figure ?: Flow cytometry results of the U2-OS Standard plate (1st measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the U2-OS cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above.The standard deviations of the replicates are also shown.]] |
|} | |} | ||
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== 9-25-2009 == | == 9-25-2009 == | ||
* We measured the MCF-7 Standard plate, which includes the same samples as above (see table 1) | * We measured the MCF-7 Standard plate, which includes the same samples as above (see table 1) | ||
- | [[image:HD09_2509_MCF7.png|center|440px|thumb|'''Figure ?: Flow cytometry results of the MCF-7 Standard plate (1st measurement).''']] | + | [[image:HD09_2509_MCF7.png|center|440px|thumb|'''Figure ?: Flow cytometry results of the MCF-7 Standard plate (1st measurement).'''The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the MCF-7 cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above.The standard deviations of the replicates are also shown.]] |
== 9-28-2009 == | == 9-28-2009 == | ||
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|-valign="top" border="0" | |-valign="top" border="0" | ||
|width="450px" style="padding: 0 20px 0 0;"| | |width="450px" style="padding: 0 20px 0 0;"| | ||
- | [[image:HD09_2809_U2os.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the U2-OS standard plate''' The .]] | + | [[image:HD09_2809_U2os.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the U2-OS standard plate(2nd measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the U2-OS cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above.The standard deviations of the replicates are also shown.]] |
|width="450px"| | |width="450px"| | ||
- | [[image:HD09_2809_MCF7.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the MCF-7 standard plate''' The .]] | + | [[image:HD09_2809_MCF7.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the MCF-7 standard plate(2nd measurement).'''The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the MCF-7 cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above.The standard deviations of the replicates are also shown.]] |
|} | |} | ||
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|-valign="top" border="0" | |-valign="top" border="0" | ||
|width="400px" style="padding: 0 20px 0 0;"| | |width="400px" style="padding: 0 20px 0 0;"| | ||
- | [[image:HD09_2909_U2os.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the U2-OS Standard plate ( | + | [[image:HD09_2909_U2os.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the U2-OS Standard plate (3rd measurement).'''The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the U2-OS cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above.The standard deviations of the replicates are also shown.]] |
|width="400px"| | |width="400px"| | ||
- | [[image:HD09_2909_MCF7.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the MCF-7 Standard plate ( | + | [[image:HD09_2909_MCF7.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the MCF-7 Standard plate (3rd measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the MCF-7 cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above. The standard deviations of the replicates are also shown.]] |
|} | |} | ||
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* We measured the constitutive promoters in the HeLa cell line again. | * We measured the constitutive promoters in the HeLa cell line again. | ||
- | [[image: | + | [[image:HD09_|center|800px|thumb|'''Figure ?: Flow cytometry results of the constitutive promoters in HeLa (1st measurement).''' .]] |
== 10-02-2009 == | == 10-02-2009 == | ||
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|-valign="top" border="0" | |-valign="top" border="0" | ||
|width="400px" style="padding: 0 20px 0 0;"| | |width="400px" style="padding: 0 20px 0 0;"| | ||
- | [[image:HD09_0510_Hela_1.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (1st measurement).''' The .]] | + | [[image:HD09_0510_Hela_1.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (1st measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the HeLa cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above. The standard deviations of the replicates are also shown.]] |
|width="400px"| | |width="400px"| | ||
- | [[image:HD09_0510_Hela_2.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (2nd measurement).''' The .]] | + | [[image:HD09_0510_Hela_2.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (2nd measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the HeLa cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above. The standard deviations of the replicates are also shown.]] |
|} | |} | ||
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|-valign="top" border="0" | |-valign="top" border="0" | ||
|width="400px" style="padding: 0 20px 0 0;"| | |width="400px" style="padding: 0 20px 0 0;"| | ||
- | [[image:HD09_0510_Hela_3.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (3rd measurement).''' The .]] | + | [[image:HD09_0510_Hela_3.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (3rd measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the HeLa cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above. The standard deviations of the replicates are also shown.]] |
|} | |} | ||
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|-valign="top" border="0" | |-valign="top" border="0" | ||
|width="400px" style="padding: 0 20px 0 0;"| | |width="400px" style="padding: 0 20px 0 0;"| | ||
- | [[image:HD09_0910_Hela_stand.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (4th measurement).''' The .]] | + | [[image:HD09_0910_Hela_stand.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (4th measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the HeLa cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above. The standard deviations of the replicates are also shown.]] |
|width="400px"| | |width="400px"| | ||
[[image:.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the SREBP responsive promoters in HeLa (1st measurement).''' The .]] | [[image:.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the SREBP responsive promoters in HeLa (1st measurement).''' The .]] | ||
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|-valign="top" border="0" | |-valign="top" border="0" | ||
|width="400px" style="padding: 0 20px 0 0;"| | |width="400px" style="padding: 0 20px 0 0;"| | ||
- | [[image:HD09_1210_Hela_stand.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (5th measurement).''' The .]] | + | [[image:HD09_1210_Hela_stand.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the HeLa Standard plate (5th measurement).''' The three standard promoters CMV, JeT, and JeT_CMV were measured 20 hours after transfection of the HeLa cells and their Relative Expression Units (REU) normalized to the JeT promoter are shown above. The standard deviations of the replicates are also shown.]] |
|width="400px"| | |width="400px"| | ||
[[image:HD09_1210_U2os_cross.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the NIIL10 promoter (NFkB responsive promoter) under different conditions in U2-OS.''' The .]] | [[image:HD09_1210_U2os_cross.png|center|400px|thumb|'''Figure ?: Flow cytometry results of the NIIL10 promoter (NFkB responsive promoter) under different conditions in U2-OS.''' The .]] |
Revision as of 13:21, 15 October 2009
Notebook Flow Cytometry9-21-2009
9-24-2009
9-25-2009
9-28-2009
9-29-2009
9-30-2009
10-02-2009
10-03-2009
10-05-2009
10-07-2009
10-08-2009
10-09-2009
10-11-2009
10-12-2009
10-13-2009
10-14-2009
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