EPF-Lausanne/14 October 2009

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==Wet Lab==
==Wet Lab==
-
Transformation of LOVTAP in iGEM plasmid worked --> Check with ColonyPCR & agarose gel
 
-
==People in the lab==
+
Transformation of LOVTAP in iGEM plasmid worked --> Check if the insert is correct with Colony PCR & agarose gel
 +
Also: our negative control worked (no clones grew)
 +
 +
 +
 +
'''Dh5-alpha RO2.4+BB1 n°3'''
 +
 +
 +
 +
We redid the initial experiment with these cells: incubated them for about 2h30 in 4 different conditions:
 +
 +
- +light+IPTG-Trp
 +
 +
- -light+IPTG-Trp
 +
 +
- -light-IPTG+Trp
 +
 +
- -light-IPTG-Trp
 +
 +
The results are the following:
 +
 +
[[Image:141009_dh5_ro2dt_2h30.jpg‎|center|thumb|upright=4|RO2 double-transformants in DH5-alpha]]
 +
 +
 +
 +
'''RO1.1 + BB1 JRG1046 cells'''
 +
 +
 +
 +
Did an experiment on the new double-transformants for RO1 in the TrpR-mutated strains: 0.5 mL of overnight culture in 3.5 mL of fresh LB. Since we had 4 different clones, for 3 of them we did only the +light+IPTG and -light+IPTG conditions, and for the 4th one we added the -light-IPTG+/-Trp conditions. The cells were exposed to conditions during about 2h before we took the measurements of OD and fluorescence with the plate reader:
 +
 +
[[Image:141009_jrg1046ro1dt_2h_multclone.jpg‎|center|thumb|upright=4|RO1 double-transformants in JRG 1046]]
 +
 +
[[Image:141009_jrg1046ro1dt_2h_clone2.jpg‎|center|thumb|upright=4|RO1 double-transformants in JRG 1046]]
 +
 +
==People in the lab==
 +
Gabriela, Tú
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Latest revision as of 11:27, 17 October 2009



Contents

14 October 2009




Wet Lab

Transformation of LOVTAP in iGEM plasmid worked --> Check if the insert is correct with Colony PCR & agarose gel

Also: our negative control worked (no clones grew)


Dh5-alpha RO2.4+BB1 n°3


We redid the initial experiment with these cells: incubated them for about 2h30 in 4 different conditions:

- +light+IPTG-Trp

- -light+IPTG-Trp

- -light-IPTG+Trp

- -light-IPTG-Trp

The results are the following:

RO2 double-transformants in DH5-alpha


RO1.1 + BB1 JRG1046 cells


Did an experiment on the new double-transformants for RO1 in the TrpR-mutated strains: 0.5 mL of overnight culture in 3.5 mL of fresh LB. Since we had 4 different clones, for 3 of them we did only the +light+IPTG and -light+IPTG conditions, and for the 4th one we added the -light-IPTG+/-Trp conditions. The cells were exposed to conditions during about 2h before we took the measurements of OD and fluorescence with the plate reader:

RO1 double-transformants in JRG 1046
RO1 double-transformants in JRG 1046

People in the lab

Gabriela, Tú


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