Team:TUDelft/24 August 2009

From 2009.igem.org

(Difference between revisions)
(Daniel)
Line 12: Line 12:
Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR
Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR
   
   
-
[[Image:Gel240809.jpg|600px]]
+
[[Image:Gel240809.jpg|550px]]

Revision as of 12:13, 19 October 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

24 August 2009

Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 kolonies

Daniel

Digestion of assemblies in order to do the second round of assembly. Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR

Gel240809.jpg


Right Gel (2%)

Well Assembly Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
2 4A 824  ?
3 5A 1040
4 2A 214  ?
5 3A 183  ?
6 1A 1076  ?
7 PCR 1
8 PCR 2
9 PCR 3
10 PCR 4
11 PCR 5
12 PCR 6

Prepare IPTG 1M for further induction. I tried to do an experiment with 1A which is GFP under control of PLacI however the culture glows even though there is no IPTG induction.

We got the oligo for the two locks and keys I designed.