Team:Todai-Tokyo/Protocols/Notebook Sample
From 2009.igem.org
(Difference between revisions)
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<h2> Gel Purification </h2> | <h2> Gel Purification </h2> | ||
<h2> RE Digest </h2> | <h2> RE Digest </h2> | ||
+ | <h2> Colony PCR </h2> |
Revision as of 02:44, 20 October 2009
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MiniprepThe following were miniprepped:
PCRThe yqiT gene was PCR amplified from pLacI-yqiT-dterm (miniprepped on 9/7) using the following primers: yqiT fwd: agcccgtgtagtactgtagagtt using PCR program 1 and Ex-taq. This PCR reaction attaches a Biobrick prefix and suffix to the gene.
Sequencing
TransformationLigationInfusionGel PurificationRE DigestColony PCR |