Team:Kyoto/CiC/Notebook/0928-1002

From 2009.igem.org

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==0928-1004 : what to do. what to do. what to do. what to do. ==
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==0928-1004 : constructing sig-GFP generator and sig-EGFP generator(HeLa) ==
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Revision as of 14:30, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0928-1004 : constructing sig-GFP generator and sig-EGFP generator(HeLa)


Monday, 28 September

To Do

  • sig-EGFP generator(HeLa)
    • Restriction Enzyme Digestion(Hind3)

Results

They were unexpectedly digested by Hind3,the RE which would not work if the signal sequence were inserted in the vector correctly.



Tuesday, 29 September

To Do

  • sig-GFP generator
    • PCR(the second PCR)
  • sig-EGFP generator(HeLa)
    • phosphorylation signal sequence primer
    • annealing signal sequence primer
    • Restriction Enzyme Digestion(EcoR1 + Xho1)
    • ligation
    • transformation

Results

No colony was observed (090930)



Wednesday, 30 September

To Do

  • sig-GFP generator
    • miniprep I712074(T7 promoter)
    • Restriction Enzyme Digestion

Results



Thursday, 01 October

To Do

Results



Friday, 02 October

To Do

Results



Saturday, 03 October

To Do

Results



Saturday, 04 October

To Do

Results



Sunday, 05 October

To Do

Results