Team:LCG-UNAM-Mexico/Wet Lab/Objectives
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* I must create an eternal bacterial source of P4 sid1. Therefore I must Infect C1a with it. | * I must create an eternal bacterial source of P4 sid1. Therefore I must Infect C1a with it. | ||
*I need to develop a good protocol for P4 sid1 lysate production. | *I need to develop a good protocol for P4 sid1 lysate production. | ||
- | * I must obtain the essential region of P4 | + | * I must obtain the essential region of P4 sid1 by PCR. This will add the preffix and suffix to the region.<br> |
3) I need to ligate the "biobricked" P4 region to a reporter; better off RFP or a hard-resistance antibiotic.<br> Then, I have to transform to produce P4 (preferably with our stock producing bacterium) and infect wild-type strains to look for reporter associated colonies. <br>(Also try in many bacterial strains)<br> | 3) I need to ligate the "biobricked" P4 region to a reporter; better off RFP or a hard-resistance antibiotic.<br> Then, I have to transform to produce P4 (preferably with our stock producing bacterium) and infect wild-type strains to look for reporter associated colonies. <br>(Also try in many bacterial strains)<br> | ||
4) When the kamikaze system is ready, I need to ligate the P4sid1 region to it and transform an E.coli C1a strain with it. <br> | 4) When the kamikaze system is ready, I need to ligate the P4sid1 region to it and transform an E.coli C1a strain with it. <br> |
Revision as of 21:25, 21 October 2009