Team:LCG-UNAM-Mexico/Wet Lab/Objectives
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In order to produce a grate amount of P4 phage particles that has our death system, we want to avoid the natural | In order to produce a grate amount of P4 phage particles that has our death system, we want to avoid the natural | ||
- | early lysis that occur when WT P4 and P2 interact. This avoidance will be achieved by taking the control of the | + | early lysis that occur when WT P4 and P2 interact. This avoidance will be achieved by taking the control of the |
- | major regulators of P2 morphopoietic genes. The control systems that is going to be implemented is constituted | + | two major regulators of P2 morphopoietic genes. The control systems that is going to be implemented is constituted |
- | promoter inducible by IPTG () in conjunction with transactivators cox and ogr from phage P2. All this will | + | by a promoter inducible by IPTG () in conjunction with transactivators cox and ogr from phage P2. All this will |
- | us to grow bacteria in grate quantities and induce lysis when ever we want and as a consequence obtation of | + | allow us to grow bacteria in grate quantities and induce lysis when ever we want and as a consequence obtation of |
- | amounts of P4 phage. | + | grate amounts of P4 phage. |
** Qualitative characterization of multipromter. | ** Qualitative characterization of multipromter. |
Revision as of 21:33, 21 October 2009