Team:IIT Bombay India/Notebook

From 2009.igem.org

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[[Week1]] FACS Trial
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{| style="color:#000000;background-color:#ffffff;" cellpadding="6" cellspacing="3" border="0" bordercolor="#ffffff" width="65%" align="center"
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[[Week2]] (May, 18-23)  Preparation
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!align="center"|[[Team:IIT_Bombay_India|Home]]
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!align="center"|[[Team:IIT_Bombay_India/Team|The Team]]
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!align="center"|[[Team:IIT_Bombay_India/Project|The Project]]
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!align="center"|[[Team:IIT_Bombay_India/Analysis|Analysis]]
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!align="center"|[[Team:IIT_Bombay_India/Modeling|Modeling]]
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!align="center"|[[Team:IIT_Bombay_India/Notebook|Notebook]]
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!align="center"|[[Team:IIT_Bombay_India/Safety|Safety]]
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|}
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[[Week3]] (May, 25-30)  FACS
 
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[[Week4]] (Jun, 1-6)    Growth Curve
 
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[[Week5]] (Jun, 8-13)    FACS:YFP signal
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[[Image:IITB-Home.jpg]]
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[[Week6]] (Jun, 15-20)  FACS:CFP signal
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{| background-color:#ffffff;" cellpadding="1.5" cellspacing="2" border="0" bordercolor="#ffffff" width="90%" align="center"
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!align="left"|
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[[Week7]] (Jun, 22-27)  FACS repeat
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|
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[[Week8]] (Jun-Jul, 29-4)Req.:Transformation
 
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[[Week9]] (Jul, 6-11)    Cultured Host
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[[Week1]] -FACS Trial
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[[Week10]](Jul, 13-18)   Transformation of 4 strains
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[[Week2]] - (May, 18-23)     Preparation
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[[Week11]](Jul, 20-24)   FACS:Constructs
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[[Week3]] - (May, 25-30)     FACS  
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[[Week12]](Jul, 27-31)   FACS
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[[Week4]] - (Jun, 1-6)       Growth Curve
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[[Week13]](Aug, 3-8)    Transformations in ''E.coli'' MG1655 (lac I deleted)  
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[[Week5]] - (Jun, 8-13)     FACS:YFP signal
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[[Week14]](Aug, 10-15)   Preparation for time course expression analysis
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[[Week6]] - (Jun, 15-20)     FACS:CFP signal
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[[Week15]](Aug, 17-22)   FACS time course
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[[Week7]] - (Jun, 22-27)     FACS repeat
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[[Week16]](Aug, 24-29)   FACS: different IPTG conc.  
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[[Week8]] - (Jun-Jul, 29-4) Req.:Transformation
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[[Week17]](Aug-Sep, 31-5)Growth curves for all the four strains
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[[Week9]] - (Jul, 6-11)     Cultured Host
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[[Week18]](Sep, 7-13)   Plasmid extraction and Gel electrophoresis
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[[Week10]]- (Jul, 13-18)     Transformation of 4 strains
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[[Week19]](Sep, 14-20)  Growth curve on Lactose (ptet A and plac K)  
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[[Week11]]- (Jul, 20-24)     FACS:Constructs
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[[Week20]](Sep, 21-27)  Growth curve on Lactose (plac A and ptet K)  
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[[Week12]]- (Jul, 27-31)     FACS
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[[Week21]](Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
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[[Week13]]- (Aug, 3-8)       Transformations in ''E.coli'' MG1655 (lac I deleted)  
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[[Week22]](Oct, 5-10)    Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
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[[Week14]]- (Aug, 10-15)     Confirmation of the transformants
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[[Week23]](Oct, 12-17)Growth curve on Lactose (ptet A and plac K)  
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[[Week15]]- (Aug, 17-22)     Preparation for time course expression analysis
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[[Week24]](Oct, 18-21)Data compilation and analysis, wiki uploads
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[[Week16]]- (Aug, 24-29)    FACS time course
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[[Week17]]- (Aug-Sep, 31-5)  FACS: different IPTG conc.
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[[Week18]]- (Sep, 7-13)      Growth curves for all the four strains 
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[[Week19]]- (Sep, 14-20)    Growth curve on Lactose (ptet A and plac K)
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[[Week20]]- (Sep, 21-27)    Growth curve on Lactose (plac A and ptet K)
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[[Week21]]- (Sep-Oct, 28-3)  Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
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[[Week22]]- (Oct, 5-10)      Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
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[[Week23]]- (Oct, 12-17)    Growth curve on Lactose (ptet A and plac K)
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[[Week24]]- (Oct, 18-21)     Data compilation and analysis, wiki uploads

Latest revision as of 22:01, 21 October 2009

Home The Team The Project Analysis Modeling Notebook Safety


IITB-Home.jpg


Week1 -FACS Trial

Week2 - (May, 18-23) Preparation

Week3 - (May, 25-30) FACS

Week4 - (Jun, 1-6) Growth Curve

Week5 - (Jun, 8-13) FACS:YFP signal

Week6 - (Jun, 15-20) FACS:CFP signal

Week7 - (Jun, 22-27) FACS repeat

Week8 - (Jun-Jul, 29-4) Req.:Transformation

Week9 - (Jul, 6-11) Cultured Host

Week10- (Jul, 13-18) Transformation of 4 strains

Week11- (Jul, 20-24) FACS:Constructs

Week12- (Jul, 27-31) FACS

Week13- (Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)

Week14- (Aug, 10-15) Confirmation of the transformants

Week15- (Aug, 17-22) Preparation for time course expression analysis

Week16- (Aug, 24-29) FACS time course

Week17- (Aug-Sep, 31-5) FACS: different IPTG conc.

Week18- (Sep, 7-13) Growth curves for all the four strains

Week19- (Sep, 14-20) Growth curve on Lactose (ptet A and plac K)

Week20- (Sep, 21-27) Growth curve on Lactose (plac A and ptet K)

Week21- (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)

Week22- (Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)

Week23- (Oct, 12-17) Growth curve on Lactose (ptet A and plac K)

Week24- (Oct, 18-21) Data compilation and analysis, wiki uploads

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