SNOWDRIFT/Proof of concept

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Proof of Concept:
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<p><span style="font-weight:bold; font-size:200%; color:#0000cc;">Proof of Concept</span></p>
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In order to establish cell-cell communication protein signalling can come in handy.
In order to establish cell-cell communication protein signalling can come in handy.
We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.
We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.
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We tried to test this export mechanism using the following biobricks:
We tried to test this export mechanism using the following biobricks:
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Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.
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<p><a href="https://2009.igem.org/SNOWDRIFT/our_idea
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Latest revision as of 02:33, 22 October 2009





Proof of Concept


In order to establish cell-cell communication protein signalling can come in handy. We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.

This tag is designed by using N-terminal sequences from proteins which are exported using the TAT Pathway.

We tried to test this export mechanism using the following biobricks:

Snowdriftproofofconcept.JPG


Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.

Next