Team:Kyoto/GSDD/Parts
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#[[Team:Kyoto/GSDD|GSDD]] | #[[Team:Kyoto/GSDD|GSDD]] | ||
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We designed following three vectors and registered intermediate products of Timer vector and constitutive LacI generator optimized in yeast. | We designed following three vectors and registered intermediate products of Timer vector and constitutive LacI generator optimized in yeast. | ||
- | ==Timer Vector for functional measurement experiment== | + | ===Timer Vector for functional measurement experiment=== |
- | This part is designed for our functional measurement of Timer vector. It consists of four part (see figure); both ends of repeated sequence of LacI binding site, constitutive eGFP generator (pTEF2-kozac-eGFP) with degradation promoted sequence (PEST), yeast centromeric sequence (CEN), yeast replication origin (ARS), constitutive uracil generator (pTEF2-kozac-URA3) with SV40 nuclear localization sequences and two ADH1 terminators (SV40-ADH1). As mentioned in Mechanism, Timer vector is a linear DNA and inserted into pSB1A2. When we use this, we can cut out linear Timer vector with a pair of restriction enzyme frequently used in iGEM, (EcoRI or XbaI)and(SpeI or PstI).[[Image:figure(timer).png|740px|center|Fig.1]] | + | This part is designed for our functional measurement of Timer vector. It consists of four part (see figure); both ends of repeated sequence of LacI binding site, constitutive eGFP generator (pTEF2-kozac-eGFP) with degradation promoted sequence (PEST), yeast centromeric sequence (CEN), yeast replication origin (ARS), constitutive uracil generator (pTEF2-kozac-URA3) with SV40 nuclear localization sequences and two ADH1 terminators (SV40-ADH1). As mentioned in Mechanism, Timer vector is a linear DNA and inserted into pSB1A2. When we use this, we can cut out linear Timer vector with a pair of restriction enzyme frequently used in iGEM, (EcoRI or XbaI)and(SpeI or PstI).[[Image:figure(timer)2.png|740px|center|Fig.1]] |
- | ==Constitutive LacI generator== | + | ===Constitutive LacI generator=== |
- | This part is also designed for our experiment. It consists of constitutive LacI generator (pTEF2-kozac-LacI) with SV40 nuclear localization sequences and two ADH1 terminators (SV40-ADH1). This part is also inserted into | + | This part is also designed for our experiment. It consists of constitutive LacI generator (pTEF2-kozac-LacI) with SV40 nuclear localization sequences and two ADH1 terminators (SV40-ADH1). This part is also inserted into BYP5005(national bioresource project).[[Image:Figure(lacI_generator)2.png|300px|center|Fig.1]] |
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Latest revision as of 02:55, 22 October 2009
Parts
Parts Design
We designed following three vectors and registered intermediate products of Timer vector and constitutive LacI generator optimized in yeast.