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| | <center> | | <center> |
| | <a href="https://2009.igem.org/Team:VictoriaBC">HOME</a> | | <a href="https://2009.igem.org/Team:VictoriaBC">HOME</a> |
| | </td> | | </td> |
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| - | <td width="10" rowspan=10 bgcolor="#163216">
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| - | <td width="750" rowspan=30 bgcolor="#e4e4fe" valign="top">
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| - | TESTING<br>
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| - | <br>
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| - | <p>Disappointing results from the flourescence microscope, none of the temperature sensitive parts were working, however the lac regulated GFP (K235032) was. The interesting thing is that the ones with IPTG seemed to have slightly less flourescence than the ones without, which is backwards to our expectations.</p><br>
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| - | <table>
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| - | <tr>
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| - | <td>Temperature</td>
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| - | <td>Room temperature</td>
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| - | <td>30°C</td>
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| - | <td>34°C</td>
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| - | <td>37°C</td>
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| - | <td>42°C</td>
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| - | <td> </td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235032 A expected result</td>
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| - | <td>g</td>
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| - | <td>g</td>
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| - | <td>g</td>
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| - | <td>g</td>
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| - | <td>g</td>
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| - | <td>This should be repressed (limited green) without lactose or IPTG independent of temperature.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235032 A actual result</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>These were quite green.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235032 B + IPTG expected</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>This should be induced by IPTG and produce green independent of temperature.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235032 B +IPTG actual</td>
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| - | <td>g</td>
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| - | <td>g</td>
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| - | <td>g</td>
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| - | <td>g</td>
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| - | <td>g</td>
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| - | <td>These were green.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235033 A expected</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>Negative control to account for microscope artifacts - there is no colour producing part at all.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235033 A actual</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>This is as expected.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235036 A expected</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>R</td>
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| - | <td>R</td>
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| - | <td>R</td>
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| - | <td>This should be controlled by the 32°C thermometer and red should be repressed under 32°C.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235036 A actual</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>No flourescence.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235037 A expected</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>This should be controlled by the 32°C thermometer and green should be repressed under 32°C.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235037 A actual</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>No flourescence. </td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235005 A expected</td>
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| - | <td>R</td>
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| - | <td>R</td>
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| - | <td>R</td>
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| - | <td>R</td>
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| - | <td>R</td>
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| - | <td>This has no promoter but might produce limited red independent of temperature.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>K235005 A actual</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>No flourescence. </td>
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| - | </tr>
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| - | <tr>
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| - | <td>J04630 expected</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>G</td>
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| - | <td>This has no promoter but might produce limited green independent of temperature.</td>
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| - | </tr>
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| - | <tr>
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| - | <td>J04630 actual</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>/</td>
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| - | <td>No flourescence. </td>
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | </tr>
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| | <tr> | | <tr> |
| | <td width="210" height="10" bgcolor="#163216"> | | <td width="210" height="10" bgcolor="#163216"> |
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| | </td> | | </td> |
| | </tr> | | </tr> |
| | + | </table> |
| | + | </td> |
| | | | |
| | + | <td width="10" rowspan=10 bgcolor="#163216"> |
| | + | <td width="750" rowspan=30 bgcolor="#e4e4fe" valign="top"> |
| | + | <b>TESTING</b><br> |
| | + | <br> |
| | + | One set of K235032 A, K235033 A, K235036 A, K235037 A, K235005, J04630, and K235032 B + IPTG, were placed in 30, 34, 37 and 42°C (non-shaking) incubation overnight. The next day, fluorescence microscopy was used for both RFP and GFP. |
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| - | | + | </td> |
| | + | </tr> |
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