EPF-Lausanne/27 July 2009
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(New page: ===Wet Lab=== LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert. [http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)] was digest...) |
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+ | <font size="6" color="#007CBC"><i>27 July 2009</i></font> | ||
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+ | ---- | ||
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+ | ==Wet Lab== | ||
LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert. | LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert. | ||
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:Gab, Christian, Mélanie, Nicolas, Nath | :Gab, Christian, Mélanie, Nicolas, Nath | ||
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+ | <a href="https://2009.igem.org/EPF-Lausanne/28_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html> | ||
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Latest revision as of 09:02, 28 July 2009
Contents |
Wet Lab
LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert.
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)] was digested, runned on a gel (which displayed the correct band) and extracted with the gel extraction kit, this step didn't worked well.
LOVTAP gene have been than digested, purified and runned on a gel.
We already had a digested [http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)].
Finally we ligated overnight at 4°C the digested LOVTAP in both [http://partsregistry.org/Part:BBa_B0015 terminators], the one digested-extracted today and the one already digested-extracted.
People in the lab
- Gab, Christian, Mélanie, Nicolas, Nath