EPF-Lausanne/28 July 2009

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<font size="6" color="#007CBC"><i>28 July 2009</i></font>
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==Wet Lab==
==Wet Lab==
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LacI, RBS and the death cassette were digested with E/S, X/P and E/P respectively. (Incubated at 37°C for 1h30) The digestion products have been purified, and than ligated (incubation at RT for 3 hours).
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The digestion products have been runned on a gel to see if the digestion worked, it worked for the death cassette but we are not able to see the LacI bands and the RBS bands.
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LOVTAP-term ligation products and LacI-RBS ligation products have been transformed in DH5-alpha.
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Finally RBS plasmid has been retransformed to have RBS part again.
==People in the lab==
==People in the lab==
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:Gab, Christian, Mélanie, Nath
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Latest revision as of 09:26, 29 July 2009



Contents

28 July 2009



Wet Lab

LacI, RBS and the death cassette were digested with E/S, X/P and E/P respectively. (Incubated at 37°C for 1h30) The digestion products have been purified, and than ligated (incubation at RT for 3 hours).

The digestion products have been runned on a gel to see if the digestion worked, it worked for the death cassette but we are not able to see the LacI bands and the RBS bands.

LOVTAP-term ligation products and LacI-RBS ligation products have been transformed in DH5-alpha.

Finally RBS plasmid has been retransformed to have RBS part again.

People in the lab

Gab, Christian, Mélanie, Nath



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