Team:EPF-Lausanne/Last News
From 2009.igem.org
(Difference between revisions)
Line 5: | Line 5: | ||
[[Image:Lov.png|lov]] | [[Image:Lov.png|lov]] | ||
[[Image:Lov2.png|lov2]] | [[Image:Lov2.png|lov2]] | ||
+ | <SCRIPT LANGUAGE="JavaScript"> | ||
+ | <!-- Begin | ||
+ | loadImage1 = new Image(); | ||
+ | loadImage1.src = "https://static.igem.org/mediawiki/2009/1/11/Lov2.png"; | ||
+ | staticImage1 = new Image(); | ||
+ | staticImage1.src = "https://static.igem.org/mediawiki/2009/4/42/Lov.png"; | ||
+ | |||
+ | // End --> | ||
+ | </script> | ||
+ | |||
+ | <!-- STEP TWO: Insert this code into the BODY of your HTML document --> | ||
+ | |||
+ | <span onmouseover="image1.src=loadImage1.src;" onmouseout="image1.src=staticImage1.src;"> | ||
+ | <img name="image1" src="https://static.igem.org/mediawiki/2009/4/42/Lov.png" border=0></span> | ||
<html><center> | <html><center> |
Revision as of 12:41, 29 July 2009
<SCRIPT LANGUAGE="JavaScript"> </script>
<img name="image1" src="" border=0>
Keep track with what we did so far
(12.07.09)
- This first week of wetlab we have done the following things
- Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
- Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
- Ordered and received the primers needed for the PCR of LovTAP
- Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
- Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
- Fused the two BioBricks "LacI" and "RBS"
- Digested the LovTAP PCR products and RBS part