Team:EPF-Lausanne/Last News

From 2009.igem.org

(Difference between revisions)
Line 7: Line 7:
-
<html><body><a>
+
<html><body>
-
<span onmouseover="image1.src=loadImage1.src;" onmouseout="image1.src=staticImage1.src;">
+
<a href="#" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('bouton','','images/bouton-vert.gif',1)"><img name="bouton" border="0" src="images/bouton-bleu_off.gif" width="187" height="29"></a>
-
<a href="https://2009.igem.org/Team:EPF-Lausanne/LOVTAP"> <img name="image1" src="https://static.igem.org/mediawiki/2009/4/42/Lov.png" border=0>
+
-
</a></span>
+
</body></html>
</body></html>

Revision as of 13:01, 29 July 2009



Contents

lov lov2




Last News



Keep track with what we did so far

(12.07.09)

This first week of wetlab we have done the following things
  • Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
  • Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
  • Ordered and received the primers needed for the PCR of LovTAP
  • Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
  • Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
  • Fused the two BioBricks "LacI" and "RBS"
  • Digested the LovTAP PCR products and RBS part


Retrieved from "http://2009.igem.org/Team:EPF-Lausanne/Last_News"