EPF-Lausanne/31 July 2009
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==Wet Lab== | ==Wet Lab== | ||
- | We transformed competent bacteria with our different ligation products from the previous day: | + | We transformed competent bacteria (DH5-alpha) with our different ligation products from the previous day: |
- neg control with vector only (Chl.) | - neg control with vector only (Chl.) | ||
- neg control without any DNA (Amp.) | - neg control without any DNA (Amp.) |
Revision as of 13:59, 2 August 2009
Contents |
People in the lab will live at the rythm of the bacteria this week end :)
Wet Lab
We transformed competent bacteria (DH5-alpha) with our different ligation products from the previous day: - neg control with vector only (Chl.) - neg control without any DNA (Amp.) - LacI-RBS from the gel extraction (Amp.) - LacI-RBS not gel-extracted (Amp.) - LacI-RBS-death cassette (Chl.)
The bacteria were left to grow overnight at 37°C on the LB-agar plates.
People in the lab
Christian, Mélanie, Nath, Basile, Gab