EPF-Lausanne/31 July 2009
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+ | People in the lab will live at the rythm of the bacteria this week end :) | ||
==Wet Lab== | ==Wet Lab== | ||
+ | |||
+ | We transformed competent bacteria (DH5-alpha) with our different ligation products from the previous day: | ||
+ | |||
+ | - neg control with vector only (Chl.) | ||
+ | |||
+ | - neg control without any DNA (Amp.) | ||
+ | |||
+ | - LacI-RBS from the gel extraction (Amp.) | ||
+ | |||
+ | - LacI-RBS not gel-extracted (Amp.) | ||
+ | |||
+ | - LacI-RBS-death cassette (Chl.) | ||
+ | |||
+ | The bacteria were left to grow overnight at 37°C on the LB-agar plates. | ||
==People in the lab== | ==People in the lab== | ||
+ | Christian, Mélanie, Nath, Basile, Gab | ||
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Latest revision as of 14:00, 2 August 2009
Contents |
People in the lab will live at the rythm of the bacteria this week end :)
Wet Lab
We transformed competent bacteria (DH5-alpha) with our different ligation products from the previous day:
- neg control with vector only (Chl.)
- neg control without any DNA (Amp.)
- LacI-RBS from the gel extraction (Amp.)
- LacI-RBS not gel-extracted (Amp.)
- LacI-RBS-death cassette (Chl.)
The bacteria were left to grow overnight at 37°C on the LB-agar plates.
People in the lab
Christian, Mélanie, Nath, Basile, Gab