EPF-Lausanne/31 July 2009

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(Wet Lab)
 
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People in the lab will live at the rythm of the bacteria this week end :)
==Wet Lab==
==Wet Lab==
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We transformed competent bacteria (DH5-alpha) with our different ligation products from the previous day:
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- neg control with vector only (Chl.)
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 +
- neg control without any DNA (Amp.)
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 +
- LacI-RBS from the gel extraction (Amp.)
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- LacI-RBS not gel-extracted (Amp.)
 +
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- LacI-RBS-death cassette (Chl.)
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The bacteria were left to grow overnight at 37°C on the LB-agar plates.
==People in the lab==
==People in the lab==
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Christian, Mélanie, Nath, Basile, Gab

Latest revision as of 14:00, 2 August 2009

Contents

31 July 2009




People in the lab will live at the rythm of the bacteria this week end :)

Wet Lab

We transformed competent bacteria (DH5-alpha) with our different ligation products from the previous day:

- neg control with vector only (Chl.)

- neg control without any DNA (Amp.)

- LacI-RBS from the gel extraction (Amp.)

- LacI-RBS not gel-extracted (Amp.)

- LacI-RBS-death cassette (Chl.)

The bacteria were left to grow overnight at 37°C on the LB-agar plates.

People in the lab

Christian, Mélanie, Nath, Basile, Gab