August/6 August 2009

From 2009.igem.org

(Difference between revisions)
(Design genetic circuits)
(Design genetic circuits)
Line 23: Line 23:
   Indcer  signal moleculer  Receiver
   Indcer  signal moleculer  Receiver
-
  LuxI ⇒     3OC6HSL      ⇒ LuxR
+
  
 +
  LuxI ⇒     3OC6HSL      ⇒ LuxR
   LasI ⇒  AI-1(3OC12HSL)  ⇒ LasR
   LasI ⇒  AI-1(3OC12HSL)  ⇒ LasR
   CinI  ⇒   3OH,C14:1-HSl   ⇒ CinR
   CinI  ⇒   3OH,C14:1-HSl   ⇒ CinR

Revision as of 21:59, 6 August 2009

Contents

Morning Meeting

To do in lab

1. Transform & Selection

  • 培地作り
    • LB
    • LB Amp+
    • LB Kan+
  • Transformation
    • About 16 kinds of parts

2. Breeding

3. Refinement

To bring

  • Timer
  • Pen
  • Slipper

Design genetic circuits

We take notice of signal molecules. So I put them in order in the chart below.

 Indcer   signal moleculer   Receiver

  

  LuxI ⇒     3OC6HSL       ⇒ LuxR
  LasI ⇒  AI-1(3OC12HSL)  ⇒ LasR
 CinI  ⇒   3OH,C14:1-HSl   ⇒ CinR