Revision as of 22:33, 6 August 2009

Morning Meeting

To do in lab

1. Transform & Selection

培地作り
- LB
- LB Amp+
- LB Kan+
Transformation
- About 16 kinds of parts

2. Breeding

3. Refinement

To bring

Timer
Pen
Slipper

Design genetic circuits

We take notice of signal molecules. So I put them in order in the chart below. 　 *Inducer ⇒（synthesize) signal moleculer ⇒（synthesize) Receiver

* LuxI ⇒　    3OC6HSL　     　⇒ LuxR
*  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
* CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR
* RhlI ⇒　　AI-1(C4HSL)  　　 ⇒　RhlR 
* （exception）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter.

 From now on,We have to grapple with the following probles.

August/6 August 2009

From 2009.igem.org

Revision as of 22:33, 6 August 2009

Contents

Morning Meeting

To do in lab

To bring

Design genetic circuits

@@ Line 20: / Line 20: @@
 ===Design genetic circuits===
-We take notice of signal molecules. So I put them in order in the chart below.<br/>
+We take notice of signal molecules. So I put them in order in the chart below.
-　                                                                                                                                 Inducer ⇒（synthesize)  signal moleculer ⇒（synthesize)   Receiver
+　                                                                                                                                 *Inducer ⇒（synthesize)  signal moleculer ⇒（synthesize)   Receiver
   * LuxI ⇒　    3OC6HSL　     　⇒ LuxR
   *  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
@@ Line 28: / Line 28: @@
   * （exception）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter.
-   From now on,We
+   From now on,We have to grapple with the following probles.