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August/10 August 2009
From 2009.igem.org
(Difference between revisions)
| Line 21: | Line 21: | ||
2-6P A | 2-6P A | ||
1-15N A | 1-15N A | ||
| + | used 8/10 competent cell | ||
Revision as of 00:08, 11 August 2009
1.Min prep
1-6O 35.2 ng/uL 1-18L 41.8 ng/uL 1-10K 39.1 ng/uL
2.competent cells(with Ca)
3.digestion with a restriction enzyme
insert : E+S vector : E+x
4.Transformation
1-23L A 1-18C A 1-20F K 1-12H K 1-6I A 2-6P A 1-15N A used 8/10 competent cell
