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August/16 August 2009
From 2009.igem.org
(Difference between revisions)
(→C4HSL P(tetR)+rhlI) |
(→AI-1 P(tetR)+lasI) |
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| Line 83: | Line 83: | ||
<tr><td>Vector</td><td>Insert</td></tr> | <tr><td>Vector</td><td>Insert</td></tr> | ||
<tr><td>RBS</td><td>LasI</td></tr> | <tr><td>RBS</td><td>LasI</td></tr> | ||
| + | <tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 BBa_C0078]</td></tr> | ||
| + | <tr><td>2091bp</td><td>609bp</td></tr> | ||
<tr><td>pSB1A2</td><td> </td></tr> | <tr><td>pSB1A2</td><td> </td></tr> | ||
</table> | </table> | ||
| Line 92: | Line 94: | ||
<tr><td>double terminator</td><td>RBS + LasI +terminator</td></tr> | <tr><td>double terminator</td><td>RBS + LasI +terminator</td></tr> | ||
<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr> | <tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr> | ||
| + | <tr><td>4554bp</td><td>654bp</td></tr> | ||
<tr><td>pSB1AK3</td><td></td></tr> | <tr><td>pSB1AK3</td><td></td></tr> | ||
</table> | </table> | ||
| Line 101: | Line 104: | ||
<tr><td>p(tetR)</td><td>RBS + LasI +terminator</td></tr> | <tr><td>p(tetR)</td><td>RBS + LasI +terminator</td></tr> | ||
<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr> | <tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr> | ||
| + | <tr><td>2091bp</td><td>609bp</td></tr> | ||
<tr><td>pSB1A2</td><td></td></tr> | <tr><td>pSB1A2</td><td></td></tr> | ||
</table> | </table> | ||
| - | |||
| - | |||
=='''3OH,C14:1-HSL''' P(tetR)+cinI<br>== | =='''3OH,C14:1-HSL''' P(tetR)+cinI<br>== | ||
Revision as of 10:48, 16 August 2009
parts planning
teble sample
| Vector | Insert |
| name | name |
| link to BBa~ | link to BBa~ |
| total size (bp) | insert size (bp) |
| Plasmid |
Contents |
sensor
signal sensor
3O6HSL P(tetR)+luxI
1.RBS + luxI
| Vector | Insert |
| RBS | luxI |
| [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | [http://partsregistry.org/Part:BBa_C0161 BBa_C0161] |
| 2091bp | 585bp |
| pSB1A2 |
2.RBS+ luxI + terminator
| Vector | Insert |
| double terminator | RBS+luxI |
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | |
| 4554bp | 579bp |
| pSB1AK3 |
3.P(tetR)+RBS+ luxI + terminator
| Vector | Insert |
| BBa_R0040 | RBS+luxI+terminator |
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |
| 2133bp | 726bp |
| pSB1A2 |
C4HSL P(tetR)+rhlI
1.RBS + rhII
| Vector | Insert |
| RBS | rhII |
| [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | [http://partsregistry.org/Part:BBa_C0170 BBa_C0170] |
| 2091bp | 609bp |
| pSB1A2 |
2.RBS + rhII + terminator
| Vector | Insert |
| double terminator | RBS + rhII |
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | |
| 4554bp | 621bp |
| pSB1AK3 |
3.P(tetR) + RBS + rhlI + terminator
| Vector | Insert |
| p(tetR) | RBS + rhII +terminator |
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |
| 2625bp | 750bp |
| pSB1A2 |
AI-1 P(tetR)+lasI
1.RBS + LasI
| Vector | Insert |
| RBS | LasI |
| [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 BBa_C0078] |
| 2091bp | 609bp |
| pSB1A2 |
2.RBS + LasI +terminator
| Vector | Insert |
| double terminator | RBS + LasI +terminator |
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | |
| 4554bp | 654bp |
| pSB1AK3 |
3.P(tetR) + RBS + LasI +terminator
| Vector | Insert |
| p(tetR) | RBS + LasI +terminator |
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |
| 2091bp | 609bp |
| pSB1A2 |
3OH,C14:1-HSL P(tetR)+cinI
1. RBS + cinI
| Vector | Insert |
| RBS | cinI |
| pSB1A2 |
2.RBS + cinI +terminator
| Vector | Insert |
| double terminator | RBS + cinI +terminator |
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | |
| pSB1AK3 |
3.P(tetR) + RBS + cinI +terminator
| Vector | Insert |
| p(tetR) | RBS + cinI +terminator |
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |
| pSB1A2 |
receiver
signal receiver
3O6HSL luxpL+luxR+luxpR
[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)
pSB1A2
C4HSL p(tetR) + rhlR + RhlR+C4
1.RhlR+p(Rhl)
| Vector | Insert |
| RhlR | p(RhlR) |
| [http://partsregistry.org/Part:BBa_I1466 BBa_I1466] | [http://partsregistry.org/Part:BBa_I14017 BBa_I14017] |
| pSB2K3 |
2.p(tetR)+RhlR+p(Rhl)
| Vector | Insert |
| p(tetR) | RhlR+p(Rhl) |
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |
| pSB1A2 |
AI-1 p(tetR) + lasR + LasR+pAI
1. RBS + lasR
| Vector | Insert |
| RBS | lasR |
| pSB1A2 |
2.RBS + lasR +terminator
| Vector | Insert |
| double terminator | RBS + lasR +terminator |
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | |
| pSB1AK3 |
3.P(tetR) + RBS + lasR +terminator
| Vector | Insert |
| p(tetR) | RBS + lasR +terminator |
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |
| pSB1A2 |
4.P(tetR) + RBS + lasR +terminator +lasR+pAI
| Vector | Insert |
| lasR+pAI | P(tetR) + RBS + lasR +terminator |
| [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | |
| pSB1A2 |
3OH,C14:1-HSL p(tetR) + cinR + cinR
1. RBS + cinR
| Vector | Insert |
| RBS | cinR |
| pSB1A2 |
2.RBS + cinR +terminator
| Vector | Insert |
| double terminator | RBS + cinR +terminator |
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | |
| pSB1AK3 |
3.P(tetR) + RBS + cinR +terminator
| Vector | Insert |
| p(tetR) | RBS + cinR +terminator |
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |
| pSB1A2 |
4.P(tetR) + RBS + cinR +terminator +p(cinR)
| Vector | Insert |
| p(cinR) | P(tetR) + RBS + cinR +terminator |
| [http://partsregistry.org/Part:BBa_R0078 BBa_R0078] | |
| pSB1A2 |
