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August/31 August 2009
From 2009.igem.org
(New page: 1.Min prep <br> filure<br> <br> 2.ligation check <br>) |
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| - | 1. | + | 1. Miniprep of (12)-B, (13)-A, (14)-A, (15)-A, (16)-A, (19)-A solution cultures inoculated from streak plates on 8/30. Failed due to mistake in procedure; the above numbered parts were again inoculated from streak plates into culture solutions for miniprep tomorrow. |
| - | + | ||
| - | + | 2. Ligation check of (12) through (16), (18) and (19) <- all miniprepped on 8/30; checking was done by taking a small sample for electrophoresis and length comparison. Results for (14), (18), (19) were not satisfactory; the others seemed okay and may be established as completed parts. | |
| - | 2. | + | |
| + | 3. Repeat of ligation assembly of (20) and (21): today their constituent parts were treated with restriction enzymes, electrophoresed and purified. Tomorrow they will be treated with ligation enzymes followed by transformation. | ||
Revision as of 15:27, 1 September 2009
1. Miniprep of (12)-B, (13)-A, (14)-A, (15)-A, (16)-A, (19)-A solution cultures inoculated from streak plates on 8/30. Failed due to mistake in procedure; the above numbered parts were again inoculated from streak plates into culture solutions for miniprep tomorrow.
2. Ligation check of (12) through (16), (18) and (19) <- all miniprepped on 8/30; checking was done by taking a small sample for electrophoresis and length comparison. Results for (14), (18), (19) were not satisfactory; the others seemed okay and may be established as completed parts.
3. Repeat of ligation assembly of (20) and (21): today their constituent parts were treated with restriction enzymes, electrophoresed and purified. Tomorrow they will be treated with ligation enzymes followed by transformation.
