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EPF-Lausanne/7 September 2009
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==Wet Lab== | ==Wet Lab== | ||
| - | + | As we got our readout1 (confirmed by PCR on miniprep + digestion assay), we have done the following test : all in LB (while waiting for a better option) | |
| + | : -Our 3 "right" clones of readout 2, induced by trp, Atc and nothing | ||
| + | : -Our 3 readout 1 (confirmed) induced by trp and nothing | ||
| + | : -A control of the "evolution of fluorescence over time" using the LacI inducible RFP from the registry that we have. This, at least, won't have any interferences. | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 07:49, 7 September 2009
Contents |
Wet Lab
As we got our readout1 (confirmed by PCR on miniprep + digestion assay), we have done the following test : all in LB (while waiting for a better option)
- -Our 3 "right" clones of readout 2, induced by trp, Atc and nothing
- -Our 3 readout 1 (confirmed) induced by trp and nothing
- -A control of the "evolution of fluorescence over time" using the LacI inducible RFP from the registry that we have. This, at least, won't have any interferences.
People in the lab
Mélanie, Caroline, Basile, Nicolas
