Team:Bologna
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The project aims to realize a device with standard biological parts | The project aims to realize a device with standard biological parts | ||
for the post-transcriptional control of gene expression, regardless of | for the post-transcriptional control of gene expression, regardless of | ||
- | the gene sequence to be silenced. | + | the gene sequence to be silenced. |
+ | <br> | ||
We designed the T-REX device, | We designed the T-REX device, | ||
composed of two non-coding DNA sequences: the TRANS-repressor and the | composed of two non-coding DNA sequences: the TRANS-repressor and the | ||
Line 67: | Line 68: | ||
of TRANS-repressor controls the translation rate of the regulated | of TRANS-repressor controls the translation rate of the regulated | ||
gene. | gene. | ||
+ | <br> | ||
The TRANS-repressor sequence was determined by a computational | The TRANS-repressor sequence was determined by a computational | ||
analysis performed to minimize the interference with the genomic mRNAs | analysis performed to minimize the interference with the genomic mRNAs | ||
and to maximize the base-pairing interaction to the CIS-repressing RNA. | and to maximize the base-pairing interaction to the CIS-repressing RNA. | ||
+ | <br> | ||
The T-REX device is proposed as a universal and fast switch in | The T-REX device is proposed as a universal and fast switch in | ||
synthetic gene circuits. | synthetic gene circuits. |
Revision as of 11:07, 28 September 2009
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Project Description
Which is our idea?
Our project aims the realization of a device for gene expression regulation at translation level, regardless of the target gene.
How can we achieve this?
We designed a 50bp non coding sequence (CIS - repressing) to be assembled upstream of the target gene RBS, while its complementary sequence (TRANS - repressor) is to be placed under the control of another promoter. The TRANS - repressing contains also a cover, that is a short sequence complementary to the RBS. When the TRANS-repressor element is present, it binds to the CIS-repressing, forming a RNA duplex and producing an obstruction that prevents the ribosome binding to the RBS.
What can we use it for?
After doing some tests to prove that the presence of CIS – repressing doesn't alter the cell normal activities, we are trying to realize some simple circuits in order to characterize our device. Several are the possible future applications, but at the moment we are aiming to oscillators and flip-flop memories.
More details about our work at the Project section.
Abstract
The project aims to realize a device with standard biological parts
for the post-transcriptional control of gene expression, regardless of
the gene sequence to be silenced.
We designed the T-REX device,
composed of two non-coding DNA sequences: the TRANS-repressor and the
CIS-repressing parts. TRANS-repressor acts as a silencer of CIS-repressing
RNA target. This target includes a region complementary to
the TRANS-repressor sequence antisense, ends with RBS, and is
assembled upstream of the coding sequence to be silenced. Upon binding
of TRANS-repressor and CIS-repressing RNAs, the access to RBS by
ribosomes is hampered, silencing translation. Accordingly, the amount
of TRANS-repressor controls the translation rate of the regulated
gene.
The TRANS-repressor sequence was determined by a computational
analysis performed to minimize the interference with the genomic mRNAs
and to maximize the base-pairing interaction to the CIS-repressing RNA.
The T-REX device is proposed as a universal and fast switch in
synthetic gene circuits.
Acknowledgements
- [http://www.unibo.it/Portale/default.htm University of Bologna]
- [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]