Team:Bologna/Wetlab
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[[Image:1429GFP_openloop_hc.png|center|700 px]] | [[Image:1429GFP_openloop_hc.png|center|700 px]] | ||
[[Image:1429GFP_openloop_lc.png|center|700 px]] | [[Image:1429GFP_openloop_lc.png|center|700 px]] | ||
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+ | PSB1A2 with high copy number plasmid and a low copy number were transformed in DH5alfa bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. One milliliter for each of the two samples was collected by O/N cultures and spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. Finally, images were elaborated with the fluorescence visualization software and these are the results: |
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To test the ratio between the production of an high copy number plasmid (PSB1A2) and a low copy number one (PSB3K3), we assembled two circuits. The open loop GFP circuits are realized with a 1429 promotor and the standard biobrick I13504.
PSB1A2 with high copy number plasmid and a low copy number were transformed in DH5alfa bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. One milliliter for each of the two samples was collected by O/N cultures and spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. Finally, images were elaborated with the fluorescence visualization software and these are the results: