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EPF-Lausanne/25 September 2009
From 2009.igem.org
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==Wet Lab== | ==Wet Lab== | ||
| + | ===Colony PCR=== | ||
| + | - 5 clones RO2#4 + BB1 JRG 1046 | ||
| + | - 4 clones RO1#1 + BB1 JW 4356-2 | ||
| + | - 1 clones RO1#1 JRG 465 | ||
| - | + | - 5 clones RO1#1 + BB1 JRG 1046 | |
| + | - Negative control | ||
| + | |||
| + | |||
| + | Numerotation of the samples : | ||
| + | |||
| + | 1 : RO1#1 + BB1 JW 4356-2 | ||
| + | |||
| + | 2 : RO1#1 JRG 456 | ||
| + | |||
| + | 3 : RO1#1 + BB1 JRG 1046 | ||
| + | |||
| + | 4 : RO2#4 + BB1 JRG 1046 | ||
| + | |||
| + | ===Counting of colonies for tests of competent cells=== | ||
| + | - JW 4356-2 : bacterial lawn -> the cells are well competent. | ||
| + | |||
| + | - JRG 1046 : 100pg : 49x4 -> 196 cells. Good efficacy : 10^6 - 10^8 | ||
| + | |||
| + | - JRG 465 : 10 pg : about 100 cells. 1ug -> 10^5 -> 10^7 so good efficacy as well. | ||
| + | |||
| + | ===Gel=== | ||
| + | We loaded samples 1,2,3,4, to see if the bands are at the right places. Conclusion : RO1#1 + BB1 are okay and RO1#1 + BB1 as well but RO1#1 and RO2#4 + BB1 didn't work. | ||
| + | |||
| + | |||
| + | ==People in the lab== | ||
| + | Gab, Nicolas | ||
Revision as of 14:27, 4 October 2009
Contents |
Wet Lab
Colony PCR
- 5 clones RO2#4 + BB1 JRG 1046
- 4 clones RO1#1 + BB1 JW 4356-2
- 1 clones RO1#1 JRG 465
- 5 clones RO1#1 + BB1 JRG 1046
- Negative control
Numerotation of the samples :
1 : RO1#1 + BB1 JW 4356-2
2 : RO1#1 JRG 456
3 : RO1#1 + BB1 JRG 1046
4 : RO2#4 + BB1 JRG 1046
Counting of colonies for tests of competent cells
- JW 4356-2 : bacterial lawn -> the cells are well competent.
- JRG 1046 : 100pg : 49x4 -> 196 cells. Good efficacy : 10^6 - 10^8
- JRG 465 : 10 pg : about 100 cells. 1ug -> 10^5 -> 10^7 so good efficacy as well.
Gel
We loaded samples 1,2,3,4, to see if the bands are at the right places. Conclusion : RO1#1 + BB1 are okay and RO1#1 + BB1 as well but RO1#1 and RO2#4 + BB1 didn't work.
People in the lab
Gab, Nicolas
