"
August/23 August 2009
From 2009.igem.org
(Difference between revisions)
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(ligation sample No.) (no. of colonies) | (ligation sample No.) (no. of colonies) | ||
| - | + | 9 1 | |
| - | + | 10 100~ | |
| - | + | 11 100~ | |
| - | + | ||
| - | + | ||
inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL) | inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL) | ||
| Line 18: | Line 16: | ||
↓<br> | ↓<br> | ||
gel electrophoresis<br> | gel electrophoresis<br> | ||
| + | [[Image:Osaka090823.jpg]]<br> | ||
| + | UP : ladder , No.8-1.2.3.4.5. , No.7-1,2,3,4,5, | ||
| + | DOWN : ladder , 1-18L , 1-23J , 1-23L , No.3 , 1-2M , 2-8G | ||
| + | <br> | ||
| + | [[Image:Osaka090823-2.jpg]] | ||
| + | ladder , 1-2M , 1-14H , 1-23L , No.6 | ||
| + | <br> | ||
gel cut<br> | gel cut<br> | ||
↓<br> | ↓<br> | ||
| Line 39: | Line 44: | ||
3.'''transfromation''' | 3.'''transfromation''' | ||
ligation sample | ligation sample | ||
| + | ⑤⑫⑬⑭⑮ | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118014 2-18H] | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R1051 1-8E] | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 1-4E] | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118006 2-16N] | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118005 2-16L] | ||
| + | |||
| + | |||
| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 08:07, 12 October 2009
1.transformed check Checked the cell cultures transformed and plated out yesterday (8/22) for colony formation and made an approximate count of the number of colonies.
(ligation sample No.) (no. of colonies)
9 1
10 100~
11 100~
inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)
2.digestion and ligation
'8/22 digestion sample
↓
gel electrophoresis
UP : ladder , No.8-1.2.3.4.5. , No.7-1,2,3,4,5, DOWN : ladder , 1-18L , 1-23J , 1-23L , No.3 , 1-2M , 2-8G
ladder , 1-2M , 1-14H , 1-23L , No.6
gel cut
↓
purification by [QIAquick Nucleotide Removal Kit]
↓
ligation
ligation DNA 44 10* buffer 5 ligation 1 total 50uL
↓
16°C 3~4hr
↓
transfromation
3.transfromation
ligation sample ⑤⑫⑬⑭⑮ [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118014 2-18H] [http://partsregistry.org/wiki/index.php?title=Part:BBa_R1051 1-8E] [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 1-4E] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118006 2-16N] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118005 2-16L]
