Team:Todai-Tokyo/Protocols/Miniprep
From 2009.igem.org
(Difference between revisions)
(New page: {{:Team:Todai-Tokyo/Template}} == Miniprep Protocol == '''Preparation''' (previous night)<BR> # Pick Single colonies from a transformation plate and culture in 4ml Luria Broth # Cultur...) |
M.Salvador (Talk | contribs) |
||
(One intermediate revision not shown) | |||
Line 1: | Line 1: | ||
{{:Team:Todai-Tokyo/Template}} | {{:Team:Todai-Tokyo/Template}} | ||
- | + | {{Team:Todai-Tokyo/protocol_temp}} | |
== Miniprep Protocol == | == Miniprep Protocol == | ||
Line 15: | Line 15: | ||
# Use the Promega miniprep kit according to instructions | # Use the Promega miniprep kit according to instructions | ||
- | We | + | We usually elute the plasmid DNA from the spin column by 50µl MilliQ and measure nucleotide concentration on a spectrophotometer to label on the tube. |
Latest revision as of 16:27, 20 October 2009
Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
---|
Miniprep Protocol
Preparation (previous night)
- Pick Single colonies from a transformation plate and culture in 4ml Luria Broth
- Culture overnight at 37ºC with vigorous shaking
Miniprep
- Aliquot 2ml of the bacterial suspension from above into a 2ml eppendorf
- Spin down for 5 min. at max speed to pellet cells
- Use the Promega miniprep kit according to instructions
We usually elute the plasmid DNA from the spin column by 50µl MilliQ and measure nucleotide concentration on a spectrophotometer to label on the tube.