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Team:Kyoto/CiC/Notebook/1005-1009
From 2009.igem.org
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{{:Team:Kyoto/CiC/Notebook_tpl}} | {{:Team:Kyoto/CiC/Notebook_tpl}} | ||
| - | ==1005-1009 : | + | ==1005-1009 : constructing sig-GFP generator,sig-EGFP generator(HeLa) == |
{{:Team:Kyoto/Pad_start_dates}} | {{:Team:Kyoto/Pad_start_dates}} | ||
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===Tuesday, 06 October=== | ===Tuesday, 06 October=== | ||
====To Do==== | ====To Do==== | ||
| - | + | No experiments were undertaken | |
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
| + | |||
===Wednesday, 07 October=== | ===Wednesday, 07 October=== | ||
====To Do==== | ====To Do==== | ||
| + | *sig-GFP generator | ||
| + | **Restriction Enzyme | ||
| + | **ligation | ||
| + | **transformation | ||
| + | *sig-EGFP generator | ||
| + | **phosphorylation signal sequence primer | ||
| + | **anneling signal sequence primer | ||
| + | **ligation | ||
| + | **transformation | ||
====Results==== | ====Results==== | ||
| + | =====Transformation===== | ||
| + | Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008) | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
===Thursday, 08 October=== | ===Thursday, 08 October=== | ||
====To Do==== | ====To Do==== | ||
| + | *sig-GFP generator | ||
| + | **Restriction Enzyme | ||
| + | **ligation | ||
| + | **transformation | ||
| + | *sig-EGFP generator | ||
| + | **phosphorylation signal sequence primer | ||
| + | **anneling signal sequence primer | ||
| + | **ligation | ||
| + | **transformation | ||
====Results==== | ====Results==== | ||
| + | =====Transformation===== | ||
| + | Sufficient,but not excessive number of colonies were observed,so we decided to move on to next step.(091010) | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
===Friday, 09 October=== | ===Friday, 09 October=== | ||
====To Do==== | ====To Do==== | ||
| - | + | *No experiments were undertaken | |
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
===Saturday, 10 October=== | ===Saturday, 10 October=== | ||
====To Do==== | ====To Do==== | ||
| + | *sig-GFP | ||
| + | **miniprep | ||
| + | **Restriction Enzyme | ||
| + | *sig-EGFP(HeLa) | ||
| + | **miniprep | ||
| + | **Restriction Enzyme | ||
====Results==== | ====Results==== | ||
| + | Plasmid construction is completed!! | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
===Saturday, 11 October=== | ===Saturday, 11 October=== | ||
====To Do==== | ====To Do==== | ||
| + | *Yeast Mitochondria isolation | ||
====Results==== | ====Results==== | ||
| + | *It is too difficult for us to isolate mitochondoria. | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
===Sunday, 12 October=== | ===Sunday, 12 October=== | ||
====To Do==== | ====To Do==== | ||
| - | + | *No experiments were undertaken | |
| - | + | ||
{{:Team:Kyoto/Pad_endof_dates}} | {{:Team:Kyoto/Pad_endof_dates}} | ||
<html></div><!-- /#rightcontents --></html> | <html></div><!-- /#rightcontents --></html> | ||
Latest revision as of 19:31, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
1005-1009 : constructing sig-GFP generator,sig-EGFP generator(HeLa)
Monday, 05 October
To Do
No experiments were undertaken
Tuesday, 06 October
To Do
No experiments were undertaken
Wednesday, 07 October
To Do
- sig-GFP generator
- Restriction Enzyme
- ligation
- transformation
- sig-EGFP generator
- phosphorylation signal sequence primer
- anneling signal sequence primer
- ligation
- transformation
Results
Transformation
Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)
Thursday, 08 October
To Do
- sig-GFP generator
- Restriction Enzyme
- ligation
- transformation
- sig-EGFP generator
- phosphorylation signal sequence primer
- anneling signal sequence primer
- ligation
- transformation
Results
Transformation
Sufficient,but not excessive number of colonies were observed,so we decided to move on to next step.(091010)
Friday, 09 October
To Do
- No experiments were undertaken
Saturday, 10 October
To Do
- sig-GFP
- miniprep
- Restriction Enzyme
- sig-EGFP(HeLa)
- miniprep
- Restriction Enzyme
Results
Plasmid construction is completed!!
Saturday, 11 October
To Do
- Yeast Mitochondria isolation
Results
- It is too difficult for us to isolate mitochondoria.
Sunday, 12 October
To Do
- No experiments were undertaken
