Team:UNICAMP-Brazil/Notebooks/October 12
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Victor.negri (Talk | contribs) (→Cre-Recombinase without ATG + terminator) |
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*<p style=”text-align:justify;”>Since yesterday's digestion didn't work, we repeated today the digestion procedure for Cre-Recombinase's biobrick. We used the same restriction enzymes, but letting the digestion last 5 hours instead of 3.</p> | *<p style=”text-align:justify;”>Since yesterday's digestion didn't work, we repeated today the digestion procedure for Cre-Recombinase's biobrick. We used the same restriction enzymes, but letting the digestion last 5 hours instead of 3.</p> | ||
*<p style=”text-align:justify;”>Then, we ran an agarose gel, in order to assure that digestion indeed happened. Unfortunately, it didn't =/</p> | *<p style=”text-align:justify;”>Then, we ran an agarose gel, in order to assure that digestion indeed happened. Unfortunately, it didn't =/</p> | ||
- | *<p style=”text-align:justify;”>After performing a few tests, we realized today that our | + | *<p style=”text-align:justify;”>After performing a few tests, we realized today that our ''Spe''I restriction enzyme was no longer capable of performing its activity. |
''Víctor'' | ''Víctor'' |
Latest revision as of 02:31, 22 October 2009
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