Team:EPF-Lausanne/Last News
From 2009.igem.org
(Difference between revisions)
Line 9: | Line 9: | ||
<a href="https://2009.igem.org/Team:EPF-Lausanne/R-BphP" onMouseOver="document.MyImage2.src='https://static.igem.org/mediawiki/2009/thumb/9/9f/Rbphp_nb.png/110px-Rbphp_nb.png';" onMouseOut="document.MyImage2.src='https://static.igem.org/mediawiki/2009/thumb/0/07/Rbphp.png/110px-Rbphp.png';"> | <a href="https://2009.igem.org/Team:EPF-Lausanne/R-BphP" onMouseOver="document.MyImage2.src='https://static.igem.org/mediawiki/2009/thumb/9/9f/Rbphp_nb.png/110px-Rbphp_nb.png';" onMouseOut="document.MyImage2.src='https://static.igem.org/mediawiki/2009/thumb/0/07/Rbphp.png/110px-Rbphp.png';"> | ||
- | <img src="https://static.igem.org/mediawiki/2009/thumb/0/07/Rbphp.png/110px-Rbphp.png" name="MyImage2"></a></center> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/0/07/Rbphp.png/110px-Rbphp.png" name="MyImage2"></a> |
+ | |||
+ | <a href="https://2009.igem.org/Team:EPF-Lausanne/Parts" onMouseOver="document.MyImage3.src='https://static.igem.org/mediawiki/2009/thumb/3/36/Parts_nb.jpg/170px-Parts_nb.jpg';" onMouseOut="document.MyImage3.src='https://static.igem.org/mediawiki/2009/thumb/e/e8/Parts.jpg/170px-Parts.jpg';"> | ||
+ | <img src="https://static.igem.org/mediawiki/2009/thumb/e/e8/Parts.jpg/170px-Parts.jpg" name="MyImage3"></a></center> | ||
</html> | </html> | ||
- | [[Image:Parts.jpg|170px]] | + | |
+ | [[Image:Parts.jpg|170px]] https://static.igem.org/mediawiki/2009/thumb/e/e8/Parts.jpg/170px-Parts.jpg | ||
[[Image:Parts_nb.jpg|170px]] https://static.igem.org/mediawiki/2009/thumb/3/36/Parts_nb.jpg/170px-Parts_nb.jpg | [[Image:Parts_nb.jpg|170px]] https://static.igem.org/mediawiki/2009/thumb/3/36/Parts_nb.jpg/170px-Parts_nb.jpg | ||
Revision as of 08:28, 30 July 2009
Keep track with what we did so far
(12.07.09)
- This first week of wetlab we have done the following things
- Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
- Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
- Ordered and received the primers needed for the PCR of LovTAP
- Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
- Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
- Fused the two BioBricks "LacI" and "RBS"
- Digested the LovTAP PCR products and RBS part