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Team:Imperial College London/Temporal Control
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* <b>Autoinduction</b>: Represses encapsulation when glucose levels are high, and kick starts it once glucose is used up. This allows a sufficient amount of protein production to have taken place before the cell focuses its resources on encapsulation. | * <b>Autoinduction</b>: Represses encapsulation when glucose levels are high, and kick starts it once glucose is used up. This allows a sufficient amount of protein production to have taken place before the cell focuses its resources on encapsulation. | ||
* <b>Thermoinduction</b>: Triggers genome deletion when the temperature is increased. Thermoinduction was necessary, as chemical induction may be blocked by the presence of the capsule (that inhibits diffusion).<br><br><br> | * <b>Thermoinduction</b>: Triggers genome deletion when the temperature is increased. Thermoinduction was necessary, as chemical induction may be blocked by the presence of the capsule (that inhibits diffusion).<br><br><br> | ||
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This testing construct was used to test the inducible promoters using flourescent proteins as output reporters.<br> | This testing construct was used to test the inducible promoters using flourescent proteins as output reporters.<br> | ||
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The timeline shows the sequence of occurrence of these events: | The timeline shows the sequence of occurrence of these events: | ||
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===Temporal Control Contents=== | ===Temporal Control Contents=== | ||
Revision as of 20:10, 15 October 2009
Temporal Control
In our project there are 3 forms of temporal control that have been implemented.
- Chemical induction: Triggers the production of the polypeptide of interest using IPTG. Effectively 'kicks off' the system once the cell density is high enough.
- Autoinduction: Represses encapsulation when glucose levels are high, and kick starts it once glucose is used up. This allows a sufficient amount of protein production to have taken place before the cell focuses its resources on encapsulation.
- Thermoinduction: Triggers genome deletion when the temperature is increased. Thermoinduction was necessary, as chemical induction may be blocked by the presence of the capsule (that inhibits diffusion).
Project Tour
For more details of the temporal control of the system, see the tabs below.
Temporal Control Contents
