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Team:Imperial College London/Temporal Control
From 2009.igem.org
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RFP is a red coloured flourescent protein that is commonly used as a reporter. The gene coding for this protein is part of the same operon as the protein of interest. As the protein of interest is produced in Module 1, RFP is coexpressed alongside. The RFP must be normalised against optical density (shown above), as the cell density is increasing throughout. | RFP is a red coloured flourescent protein that is commonly used as a reporter. The gene coding for this protein is part of the same operon as the protein of interest. As the protein of interest is produced in Module 1, RFP is coexpressed alongside. The RFP must be normalised against optical density (shown above), as the cell density is increasing throughout. | ||
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*[http://en.wikipedia.org/wiki/Green_fluorescent_protein GFP] is a green coloured flourescent protein that is also commonly used as a reporter. The coding gene is under control of the same promoter as the genes for Module 2. This means that GFP expression is tied into the module, and we can see the rise in GFP levels that correlate with the switch to the secondary carbon source and therefore the start of Module 2. The GFP again must be normalised against optical density (shown above) to account for the increase in cell density.--> | *[http://en.wikipedia.org/wiki/Green_fluorescent_protein GFP] is a green coloured flourescent protein that is also commonly used as a reporter. The coding gene is under control of the same promoter as the genes for Module 2. This means that GFP expression is tied into the module, and we can see the rise in GFP levels that correlate with the switch to the secondary carbon source and therefore the start of Module 2. The GFP again must be normalised against optical density (shown above) to account for the increase in cell density.--> | ||
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===Project Tour=== | ===Project Tour=== | ||
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<a href="https://2009.igem.org/Team:Imperial_College_London/Genetic_Circuit"><img width=150px src="https://static.igem.org/mediawiki/2009/c/ce/II09_Temp_ArrowRight.png"></a> | <a href="https://2009.igem.org/Team:Imperial_College_London/Genetic_Circuit"><img width=150px src="https://static.igem.org/mediawiki/2009/c/ce/II09_Temp_ArrowRight.png"></a> | ||
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<b><i>For more details of the temporal control of the system, see the tabs below.</i></b> | <b><i>For more details of the temporal control of the system, see the tabs below.</i></b> | ||
Revision as of 20:11, 15 October 2009
Temporal Control
In our project there are 3 forms of temporal control that have been implemented.
- Chemical induction: Triggers the production of the polypeptide of interest using IPTG. Effectively 'kicks off' the system once the cell density is high enough.
- Autoinduction: Represses encapsulation when glucose levels are high, and kick starts it once glucose is used up. This allows a sufficient amount of protein production to have taken place before the cell focuses its resources on encapsulation.
- Thermoinduction: Triggers genome deletion when the temperature is increased. Thermoinduction was necessary, as chemical induction may be blocked by the presence of the capsule (that inhibits diffusion).
Project Tour
For more details of the temporal control of the system, see the tabs below.
Temporal Control Contents
