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Team:EPF-Lausanne/Protocols/Illumination
From 2009.igem.org
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'''Material''' | '''Material''' | ||
| - | * | + | *LED blue 3mm (Distrelec) |
| - | * | + | *LED750-5mm (Roithner LaserTechnik) |
| - | * | + | *AC Power supply |
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<br> | <br> | ||
'''Procedure''' | '''Procedure''' | ||
<br> | <br> | ||
| - | |||
| - | |||
| - | |||
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| + | The initial cell culture is made in dark state. We then illuminate them in the incubator, with constant shaking. To do so, we use small erlenmeiers to have a sufficient amount of culture for the experiments and so that the cells are in a sufficient medium to grow. | ||
| + | We aslo use the red LEDs to inactivate the LovTAP while loading our samples for further experiments. | ||
</div><div CLASS="epfl09bouchon"></div> | </div><div CLASS="epfl09bouchon"></div> | ||
Revision as of 12:29, 18 October 2009
Material
- LED blue 3mm (Distrelec)
- LED750-5mm (Roithner LaserTechnik)
- AC Power supply
Procedure
The initial cell culture is made in dark state. We then illuminate them in the incubator, with constant shaking. To do so, we use small erlenmeiers to have a sufficient amount of culture for the experiments and so that the cells are in a sufficient medium to grow. We aslo use the red LEDs to inactivate the LovTAP while loading our samples for further experiments.
