Team:Todai-Tokyo/Protocols/Notebook Sample

From 2009.igem.org

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<h2> PCR </h2>
<h2> PCR </h2>
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The yqiT gene was [[Team:Todai-Tokyo/Protocols/PCR|PCR]] amplified from pLacI-yqiT-dterm ([[Team:Todai-Tokyo/Protocols/Miniprep|miniprep]]ped on 9/7) using the following primers:
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yqiT fwd: agcccgtgtagtactgtagagt
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yqiT rev: agggatgtttgccagtcgtaattag
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Using [[Team:Todai-Tokyo/Protocols/PCR|PCR program 1]]
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<h2> Sequencing </h2>
<h2> Sequencing </h2>
<h2> Transformation </h2>
<h2> Transformation </h2>

Revision as of 02:35, 20 October 2009

UT.png
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Contents

Miniprep

The following were miniprepped:

  • pLacI-RFP-dterm (From Ligation on 9/4; 4 colonies)
  • RFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] from [http://partsregistry.org/assembly/libraries.cgi?id=19 2009 Spring Distribution]; 6 colonies)

PCR

The yqiT gene was PCR amplified from pLacI-yqiT-dterm (miniprepped on 9/7) using the following primers:

yqiT fwd: agcccgtgtagtactgtagagt yqiT rev: agggatgtttgccagtcgtaattag

Using PCR program 1


Sequencing

Transformation

Ligation

Infusion

Gel Purification

RE Digest

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