Team:Imperial College London/Wetlab/Results/Cheminduction

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(Difference between revisions)
(Experiment:)
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[[Image:II09_IPTG_growth1.jpg]]<br>
[[Image:II09_IPTG_growth1.jpg]]<br>
<b>Figure 1: Effects of [IPTG] on cell growth</b>: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.<br>
<b>Figure 1: Effects of [IPTG] on cell growth</b>: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.<br>
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<br>
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Simply plotting the raw data does not give sufficient information about possible trends.
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Therefore, further analysis was performed where:
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1. The blank absorbance level was estimated
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[[Image:II09_chemo1_blank_variation.png]]
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{{Imperial/09/TemplateBottom}}
{{Imperial/09/TemplateBottom}}

Revision as of 20:17, 20 October 2009

Experiment:

Different concentrations of IPTG were added to a 96-well plate [link to assay] of e-coli Top 10 cells containing LacI-RFP genetic constructs. Here we measured the optical density of the cells over-time, for different concentrations of IPTG to monitor the effects in the culture. The IPTG growth curve results are plotted below:
II09 IPTG growth1.jpg
Figure 1: Effects of [IPTG] on cell growth: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.

Simply plotting the raw data does not give sufficient information about possible trends. Therefore, further analysis was performed where: 1. The blank absorbance level was estimated II09 chemo1 blank variation.png


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