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Team:Imperial College London/Wetlab/Results/Cheminduction
From 2009.igem.org
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1. The blank absorbance level was estimated<br> | 1. The blank absorbance level was estimated<br> | ||
[[Image:II09_chemo1_blank_variation1.png]]<br> | [[Image:II09_chemo1_blank_variation1.png]]<br> | ||
| - | <b>Figure 2: Variation of the blank well data in monitoring absorbance</b> A1, A2, B1, B2: 4 wells containing blank M9 media. | + | <b>Figure 2: Variation of the blank well data in monitoring absorbance</b> A1, A2, B1, B2: 4 wells containing blank M9 media.<br> |
| - | [[Image:II09_IPTG_growth_2.jpg|720px]] | + | [[Image:II09_IPTG_growth_2.jpg|720px]]<br> |
| - | + | <b>Figure 3: Log plots of the exponential growth phase for different IPTG concentrations </b><br> | |
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 20:40, 20 October 2009
Experiment:
Different concentrations of IPTG were added to a 96-well plate of e-coli Top 10 cells containing LacI-RFP genetic constructs. Here we measured the optical density of the cells over-time, for different concentrations of IPTG to monitor the effects in the culture. The IPTG growth curve results are plotted below:
Figure 1: Effects of [IPTG] on cell growth: Note that the high initial OD value results due to cultures having grown for 1 hour prior the start of the measuring phase.
Simply plotting the raw data does not give sufficient information about possible trends.
Therefore, further analysis was performed where:
1. The blank absorbance level was estimated
Figure 2: Variation of the blank well data in monitoring absorbance A1, A2, B1, B2: 4 wells containing blank M9 media.
Figure 3: Log plots of the exponential growth phase for different IPTG concentrations
