Team:DTU Denmark/results

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Revision as of 21:10, 20 October 2009

The project

Notebook

The redoxilator

- Genetic design
- Applications and perspectives
- Results
- Safety considerations

The USER assembly standard

- USER fusion of biobricks

USER fusion primer design software

- Abstract
- Instructions
- Output format

The project

Results

Sub header
Insert text here

Achievements

Redox sensing device
Two novel genes have been designed and synthesized each comprised of 5 genetic elements. Together they function as a device termed the Redoxilator that can sense the internal redox state of a yeast cell, and output a reporter signal.

Biobricks
DNA of several new biobricks have been designed and submitted including a yeast optimized GFP reporter protein, a protein degradation sequence and a fast degradable yeast GFP. (Bronze medal)

We have demonstrated that our USER fusion biobrick works as expected and documented it (silver medal)

USER fusion Assembly standard
A new biobrick assembly standard that allows the rapid construction of multi-part devices have been developed and documented. The assembly standard offers many benefits: All restriction sites are allowed, multiple biobricks can be joined in one step, the result is scar-free making it ideal for protein fusions and more. (Gold medal)

USER-fusion primer design software
A novel and very useful software tool have been developed that can automatically design the optimal primers for USER fusion assembly of 2-9 biobricks, taking several parameters into account.

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-   <b>Synthetic biology</b><br><br>
+   <b>Achievements</b><br><br>
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-<p align="left"><i>“Synthetic Biology is an art of engineering new biological systems that don’t exist in nature.”</i><br></p>
+<p>
+<strong>Redox sensing device</strong><br>
-<p align="right"><i>-Paras Chopra & Akhil Kamma</i><br><br></p>
+Two novel genes have been designed and synthesized each comprised of 5 genetic elements. Together they function as a device termed the Redoxilator that can sense the internal redox state of a yeast cell, and output a reporter signal.<br>
+<br>
-<p>In nature, biological molecules work together in complex systems to serve purposes of the cell. In synthetic biology these molecules are used as individual functional units that are combined to form tailored systems exhibiting complex dynamical behaviour. From ‘design specifications’ generated from computational modelling, engineering-based approaches enables the construction of such new specified gene-regulatory networks. The ultimate goal of synthetic biology is to construct systems that gain new functions, and the perspectives of the technology are enormous. It has already been used in several medical projects and is predicted to play a major role in biotech-production and environmental aspects.</p>
+<strong>Biobricks</strong><br>
+DNA of several new biobricks have been designed and submitted including a yeast optimized GFP reporter protein, a protein degradation sequence and a fast degradable yeast GFP. (Bronze medal)<br>
+<br>
+We have demonstrated that our USER fusion biobrick works as expected and documented it (silver medal)<br>
+<br>
+<strong>USER fusion Assembly standard</strong><br>
+A new biobrick assembly standard that allows the rapid construction of multi-part devices have been developed and documented. The assembly standard offers many benefits: All restriction sites are allowed, multiple biobricks can be joined in one step, the result is scar-free making it ideal for protein fusions and more. (Gold medal)<br>
+<br>
+<strong>USER-fusion primer design software</strong><br>
+A novel and very useful software tool have been developed that can automatically design the optimal primers for USER fusion assembly of 2-9 biobricks, taking several parameters into account.
+</p>