Team:UQ-Australia/Safety
From 2009.igem.org
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'''Public and Environmental Safety''' <br> | '''Public and Environmental Safety''' <br> | ||
- | No plans were made to release the organisms or DNA involved into the environment. Cleaning procedures were strictly enforced so that this did not occur. A specific issue involved with release of P. syringae is the grape industry. Currently, research is underway to knockout the ice nucleating gene of P. syringae as the organism may be detrimental to particular plants. Accidental release of a heat-resistant strain of this organism would be | + | No plans were made to release the organisms or DNA involved into the environment. Cleaning procedures were strictly enforced so that this did not occur. A specific issue involved with release of P. syringae is the grape industry. Currently, research is underway to knockout the ice nucleating gene of P. syringae as the organism may be detrimental to particular plants. Accidental release of a heat-resistant strain of this organism would not be ideal. At this point in time, P. syringae have not been used extensively in the production of registry parts (E. coli was used as a substitute).<br> |
- | Transportations of chemicals, DNA and organisms was always double-contained to prevent | + | Transportations of chemicals, DNA and organisms was always double-contained to prevent environmental contamination.<br> |
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+ | The Institutional Biosafety Committee in the Occupational Health and Safety Unit at UQ approves of the current lab practices used to protect the safety of laboratory members and to ensure that GMOS are not released into the environment. Further consideration needs to be given to the possible environmental consequences of releasing these organisms. |
Revision as of 09:40, 21 October 2009
Safety ConsiderationsResearcher Safety - Personal protective equipment (gloves, shoes, goggles, labcoat) Gel post-staining with Ethidium bromide had special precautions relating to its carcinogenic properties: - Dedicated fume cupboard where Ethidium bromide is handled. Agarose gels: - Care was taken when heating agarose solutions to dissolve agarose. Use of the UV transilluminator involved particular precautions: - Only UV glasses to be worn when viewing the gels. Bunsen burners: - Long hair tied back to prevent fire hazards. All surfaces were sprayed down with ethanol before and after experimental work. Public and Environmental Safety |