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Duke University/17 June 2009
From 2009.igem.org
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| - | + | [[Team:Duke | Return]] | |
| - | + | ||
| - | Measured concentration of DNA fragments 3 and 4 | + | ==June 17, 2009== |
| + | *E: PCR with Bioplastics #4 as template, using Phusion enzyme protocol (shorter denaturation time, lower annealing temperature), Gel to check PCRs | ||
| + | |||
| + | <pre> | ||
| + | 98°C 15s 1x | ||
| + | 98°C 1s | ||
| + | 50°C 5s | ||
| + | 72°C 30s 35x | ||
| + | 72°C 2m | ||
| + | 4°C infinity 1x | ||
| + | </pre> | ||
| + | |||
| + | *R: Correct bands | ||
| + | *C: Cut out bands | ||
| + | |||
| + | |||
| + | *E: Measured concentration of DNA fragments 3 and 4 | ||
| + | *R: Concentrations high | ||
| + | *C: Do assembly PCR | ||
Latest revision as of 16:41, 21 October 2009
June 17, 2009
- E: PCR with Bioplastics #4 as template, using Phusion enzyme protocol (shorter denaturation time, lower annealing temperature), Gel to check PCRs
98°C 15s 1x 98°C 1s 50°C 5s 72°C 30s 35x 72°C 2m 4°C infinity 1x
- R: Correct bands
- C: Cut out bands
- E: Measured concentration of DNA fragments 3 and 4
- R: Concentrations high
- C: Do assembly PCR
