Team:EPF-Lausanne/Notebook/Cloning Strategy

From 2009.igem.org

(Difference between revisions)
Nbranden (Talk | contribs)
(New page: ;Cloning strategy: Four forward primers were designed to amplify: <br> 1.Promoter T7, RBS, CBP and LOVTAP: :gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg 2.RBS, CBP and LOVT...)
Newer edit →

Revision as of 13:55, 8 July 2009

Cloning strategy

Four forward primers were designed to amplify:
1.Promoter T7, RBS, CBP and LOVTAP:

gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg

2.RBS, CBP and LOVTAP:

gtttcttcgaattcgcggccgcttctagagtgtttaactttaagaaggag

3.CBP and LOVTAP:

gtttcttcgaattcgcggccgcttctagatgaagcgacgatggaaaaagaatttcatag

4.LOVTAP:

gtttcttcgaattcgcggccgcttctagatgctactacacttgaacgtattgagaagaac

One reverse primer were designed:

gtttcttcctgcagcggccgctactagtatcaatcgcttttcagcaacacctcttc


The recipient IGEM part have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1