"
Team:Warsaw/Calendar-Main/9 July 2009
From 2009.igem.org
(Difference between revisions)
Ffijalkowski (Talk | contribs) |
|||
| Line 47: | Line 47: | ||
</html> | </html> | ||
<!-- TU PISZ CO CHCESZ! --> | <!-- TU PISZ CO CHCESZ! --> | ||
| - | + | <html> | |
| + | <h4>Jarek</h4> | ||
| + | <br> | ||
| + | <p>Task:</p> | ||
| + | <ul> | ||
| + | <li>Isolation of plasmid containing parts from liquid cultures</li> | ||
| + | <li>Digestion of acquired samples with restriction endonucleases</li> | ||
| + | <li>Electrophoretic separation of digested samples</li> | ||
| + | <li>Isolation of samples from agarose gel<li> | ||
| + | </ul> | ||
| + | <br> | ||
| + | <p>Methods:</p> | ||
| + | <ul> | ||
| + | <li>Plasmid DNA was isolated with A&A "Plasmid Mini" kit, the DNA concentration was measured with nanodrop</li> | ||
| + | <li>For digestion 1 ul of PstI/SpeI (B0032) or PstI/XbaI enzymes and 2 ul of 1xTango buffer were used. Digestion was held for 3 hours.</li> | ||
| + | <li>After digestion samples were separated due to elecrophoresis in 0,8 agarose gel in TBE buffer</li> | ||
| + | <li>Samples were isolated from gel with A&A "Gel-out" kit</li> | ||
| + | </ul> | ||
| + | <br> | ||
| + | <p>Results:</p> | ||
| + | <ul> | ||
| + | <li></li> | ||
| + | </ul> | ||
| + | </html> | ||
Revision as of 21:14, 10 July 2009
 |  |
 |
  |
 |
|
Isolation of BioBricks from 2008 and 2009 Kit Plates
Monika
Selected BioBricks:
Miecznikowa team: adjusting part BBa_J5528
Franek
Task:
- Alkaline lysis of the plasmid containing BBa_I0500
- Transform competent cells with BBa_B0024
- Second attempt to transform competent cells with BBa_I0500
Methods:
- Plates with BBa_I0500 were empty, therefore once again transformation of chemocompetent cells was performed, but this time 8ul of BBa_I0500 DNA solution was used
- Plating bacterias with BBa_I0500 on LB medium supplemented with kanamycin
- Resuspension of DNA from plate 1, 2C (BBa_B0024) with 15ul of H2O
- Transformation of chemocompetent cells with 4ul of BBa_B0024 DNA solution
- Plating bacterias with BBa_B0024 on LB medium supplemented with ampicillin
Results:
- Will be determined tomorrow
Jarek
Task:
- Isolation of plasmid containing parts from liquid cultures
- Digestion of acquired samples with restriction endonucleases
- Electrophoretic separation of digested samples
- Isolation of samples from agarose gel
Methods:
- Plasmid DNA was isolated with A&A "Plasmid Mini" kit, the DNA concentration was measured with nanodrop
- For digestion 1 ul of PstI/SpeI (B0032) or PstI/XbaI enzymes and 2 ul of 1xTango buffer were used. Digestion was held for 3 hours.
- After digestion samples were separated due to elecrophoresis in 0,8 agarose gel in TBE buffer
- Samples were isolated from gel with A&A "Gel-out" kit
Results:
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
