Team:Groningen/Brainstorm/Growth Control

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Introduction

Bacteria have a choice between using nutrients for growth or for the production of (commercially) valuable proteins. Being able to control the [http://en.wikipedia.org/wiki/Bacterial_growth bacterial growth cycle] and inducing a premature stationary phase will create the possibility to spend more time producing, using less nutrients for biomass and more for desired production. Stationary phase is nothing more than a stop in an increase of cell numbers by cell death being equal to cell growth. Normally this is induced by the limitation of available nutrients or by means of [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in response to high cell density. With this knowledge, a culture can be created that can respond by limited cell death in response to an added molecule that mimics the response to high cell density.

See also [http://en.wikipedia.org/wiki/Chemostat Chemostat]
One of the most important features of chemostats is that micro-organisms can be grown in a physiological steady state. In steady state, all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). Because obtaining a steady state requires at least 5 volume changes, chemostats require large nutrient and waste reservoirs. Creating biological "chemostat" would circumvent these drawbacks.

Previous contests

Quorum Sensing

Cell cycle

[http://parts.mit.edu/wiki/index.php/Synchronization_of_Cell_Cycles Bangalore 2006]
Strasbourg 2008

Parts in the [http://partsregistry.org/Main_Page Registry of Standard Parts]:

Quorum sensing

[http://partsregistry.org/Part:BBa_K104001 BBa_K104001]: Sensor for small peptide Subtilin
[http://partsregistry.org/Part:BBa_I13211 BBa_I13211]: Biobricked version of the natural Lux quorum sensing system

Cell cycle

[http://partsregistry.org/Part:BBa_M31201 BBa_M31201]
[http://partsregistry.org/Part:BBa_K105013 BBa_K105013]
[http://partsregistry.org/Part:BBa_K105015 BBa_K105015]
[http://partsregistry.org/Part:BBa_K101017 BBa_K101017]: a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after
[http://partsregistry.org/Part:BBa_J22051 BBa_J22051]: an adenylate cyclase promoter, expression is repressed during cell division
[http://partsregistry.org/Part:BBa_J22052 BBa_J22052]: an adenylate cyclase promoter, expression is repressed during cell division
[http://partsregistry.org/Part:BBa_J22095 BBa_J22095]
[http://partsregistry.org/Part:BBa_J22092 BBa_J22092]
[http://partsregistry.org/Part:BBa_K142040 BBa_K142040]: Ribosome modulation factor (RMF)
[http://partsregistry.org/Part:BBa_K142041 BBa_K142041]: Arabinose controlled RMF generator

Cell death

[http://partsregistry.org/Part:BBa_I745006 BBa_I745006]
[http://partsregistry.org/Part:BBa_I745007 BBa_I745007]
[http://partsregistry.org/Part:BBa_K145008 BBa_K145008]: LuxR Generator
[http://partsregistry.org/Part:BBa_K145009 BBa_K145009]: ccdB cell death gene under control of an activating Lux PR
[http://partsregistry.org/Part:BBa_K145109 BBa_K145109]: ccdB cell death gene under the control of a hybrid LuxPR P22 C2 promotor
[http://partsregistry.org/Part:BBa_K145110 BBa_K145110]: Complete cell death mechanism. Combination of [http://partsregistry.org/Part:BBa_K145108 BBa_K145108] and [http://partsregistry.org/Part:BBa_K145109 Part:BBa_K145109]
[http://partsregistry.org/Part:BBa_K145151 BBa_K145151]: Coding region for the ccdB (control of cell death) gene
[http://partsregistry.org/Part:BBa_K145230 BBa_K145230]: A hybrid promoter controls the production of LuxR and ccdB
[http://partsregistry.org/Part:BBa_K145256 BBa_K145256]: Cell death Part 1
[http://partsregistry.org/Part:BBa_K145257 BBa_K145257]: Cell death Part 2
[http://partsregistry.org/Part:BBa_K124003 BBa_K124003]: Induces lysis in E. Coli bacteria
[http://partsregistry.org/Part:BBa_K124014 BBa_K124014]: Induces lysis faster in E. Coli bacteria
[http://partsregistry.org/Part:BBa_K124017 BBa_K124017]: Complete casette containing [http://partsregistry.org/Part:BBa_K124014 BBa_K124014]

Related Literature

Quorum sensing

[http://www.ncbi.nlm.nih.gov/pubmed/15374644 Quorum sensing control of lantibiotic production; nisin and subtilin autoregulate their own biosynthesis], Kleerebezem

In this paper, the molecular mechanism underlying regulation of nisin and subtilin production is reviewed.

[http://www.ncbi.nlm.nih.gov/pubmed/9675856 Induction of entry into the stationary growth phase in Pseudomonas aeruginosa by N-acylhomoserine lactone], You et al.

Addition of N-acylhomoserine lactone in the exponential growth phase, regardless of cell density, induces a repression of cell growth of P. aeruginosa

[http://www.ncbi.nlm.nih.gov/pubmed/12032343 Induction of natural competence in Streptococcus pneumoniae triggers lysis and DNA release from a subfraction of the cell population], Steinmoen et al.

In this study Competence stimulating peptide is shown to initiates release of DNA from a subfraction of the bacterial population, probably by cell lysis.

[http://www.nature.com/msb/journal/v4/n1/full/msb200824.html A synthetic Escherichia coli predator–prey ecosystem], Balagaddé et al.

In this study they created a synthetic ecosystem with bi-directional communication through quorum sensing which regulate each other's gene expression and survival via engineered gene circuits.

@@ Line 39: / Line 39: @@
 :*[http://partsregistry.org/Part:BBa_J22095 BBa_J22095]
 :*[http://partsregistry.org/Part:BBa_J22092 BBa_J22092]
+:*[http://partsregistry.org/Part:BBa_K142040 BBa_K142040]: <i>Ribosome modulation factor (RMF)</i>
+:*[http://partsregistry.org/Part:BBa_K142041 BBa_K142041]: <i>Arabinose controlled RMF generator</i>
 <b>Cell death</b>